Citations 1
Effect of residual platelets in frozen-thawed plasma on results of dilute Russell’s viper venom time assay for lupus anticoagulant testing
Strande, Brenda J Nelson, Leger, Rachel, Stuart, Melissa S, Tange, Julie I, Navitska, Serena D, Heikal, Nahla, Ashrani, Aneel A. , Chen, Dong , Seheult, Jansen N. , Pruthi, Rajiv K. , Leger, Rachel R, Chen, Dong , Seheult, Jansen N , Sridharan, Meera
American Journal of Clinical Pathology | October 25, 2023 | Volume: 161 | Issue: 3 | Pages: 212-215
Abstract Objectives To determine the impact of residual platelets on dilute Russell’s viper venom time (DRVVT) assay in frozen-thawed plasma submitted for lupus anticoagulant (LAC) testing. Methods We measured platelet counts in frozen-thawed samples submitted for LAC testing and evaluated the association between platelet count and the DRVVT screening time and ratios. We also spiked platelets into a LAC-positive sample to observe the effect on the DRVVT. Results Progressive increase in platelet count resulted in a statistically significant shortening of the DRVVT assay results on plasma after 1 freeze-thaw cycle. A similar effect was noted on the LAC-positive sample. Conclusions Residual platelets in plasma samples result in shortening of DRVVT assay after 1 freeze-thaw cycle. This may result in a false-negative LAC test result.