Kantarci, Kejal

AbstractBackgroundSeveral programs are available for automatically “de‐facing” brain images. These are designed to prevent the potential use of face imagery to re‐identify research participants. Previous works have compared available software and mri_reface is frequently among the top methods. The “Minimally Invasive DeFacing of MRI images” (MiDeFace) program was more recently added to FreeSurfer, but minimal validation of MiDeFace has been published.MethodWe ran mri_reface 0.3.3 and MiDeFace 7.3.2 on two datasets: A) 207 participants with face photographs and 3D T1‐weighted and 3D T2‐weighted‐FLAIR MRI from the Mayo Clinic Study of Aging (all unimpaired, Siemens); and B) 300 participants from ADNI3 with 3D T1‐weighted MRI (50 unimpaired + 50 with clinical AD, from each of 3 vendors). For dataset A, we tested the rate at which Microsoft Azure face recognition matched each participant’s photographs with the correct MRI, comparing unmodified images, those from mri_reface, and those from MiDeFace. For dataset B, we compared measured regional brain volume and cortical thickness from original vs. de‐faced images with SPM12, FreeSurfer 7.4.1, and the FSL‐UK Biobank pipeline. For both datasets, images were visually rated to assess removal of the face and retention of brain tissue.ResultAutomated face recognition match rates were lower with mri_reface (17/207 8.2%) than with MiDeFace (24/207 11.6%), indicating stronger identity protection with mri_reface, but both were greatly reduced vs. unmodified images (203/207 98.1%). Example images with both programs are shown in Figure 1. From visual ratings (Table 1), rates of complete success (modification of the entire face while retaining the entire brain) were much higher with mri_reface, but the great majority of failures from MiDeFace were subtle and occurred in relatively less recognizable face regions. The effects of both programs on downstream imaging measurements (Figure 2) were small and very comparable, but MiDeFace was favored mildly for SPM12 and for FSL, while mri_reface was more favored for FreeSurfer.ConclusionAltogether, mri_reface more reliably removed the complete face, leading to smaller rates of potential re‐identification (8.2% vs 11.6%). Effects on downstream image measurements were small and comparable with both methods.

AbstractBackgroundThe Mayo Clinic Study of Aging (MCSA) is a longitudinal, population‐based study of residents of Olmsted County, Minnesota, USA. MCSA is releasing de‐identified clinical and imaging data on GAAIN.org to benefit the research community.MethodWe included longitudinal clinical data from all participants of the Mayo Clinic Study of Aging 30‐90 years of age (average 70.0y) through the first 15 years of the study. Clinical data includes age, sex, self‐reported race/ethnicity, APoE4 allele status, cognitive impairment status (normal, MCI, dementia, or other), height, weight, blood pressure, multi‐domain neuropsychological scores, neuroimaging summary measurements, medications, cardiovascular risk factors, social activity, sleep, mood/anxiety, functional measures, and more. For our initial release of imaging data, we selected the first imaging timepoint acquired on 3T GE MRI scanners and amyloid (PiB) PET during the same visit, from all available imaging participants 50‐90 years of age (average 70.7y). Currently, we included 3D T1‐weighted (MPRAGE) MRI (1x1x1.2mm3), T2‐weighted FLAIR MRI (0.85x0.85x3.6mm3), diffusion MRI (2.7mm resolution with 41 directions at b=1000 and 5 b=0), and numeric cortical global SUVR and Centiloid values from PiB PET scans at 40‐60 minutes post‐injection. All images are released in DICOM format and thoroughly de‐identified using CTP while ensuring preservation of necessary fields for analyses. T1‐weighted and T2‐FLAIR MRI were also de‐faced (identifiable face imagery was removed and replaced with an average) using mri_reface version 0.3.3 to further protect participants’ privacy, and all de‐faced images were visually inspected by a trained data scientist for quality assurance.ResultThe first phase of our public data release includes clinical data from 5925 unique participants from the Mayo Clinic Study of Aging (MCSA), each ranging from 1‐12 visits at roughly 15‐month intervals, and three MRI sequences (T1‐w, T2‐w‐FLAIR, and diffusion) from 1802 unique participants. Demographic summaries are given in Table 1.ConclusionThis large, richly characterized, de‐identified clinical and imaging dataset is available to the research community at https://www.gaaindata.org/partner/MCSA, and it is suitable for a wide range of research uses. Additional data will be released over time, including de‐faced amyloid PET images.

AbstractBackgroundMedial temporal lobe (MTL) atrophy is an early feature of multiple neurodegenerative diseases. In genetic frontotemporal lobar degeneration (FTLD, i.e. individuals carrying GRN, MAPT, or C9orf72 mutations), MTL atrophy can occur 10‐15 years before symptom onset. We aimed to better characterize MTL atrophy patterns in presymptomatic and symptomatic genetic FTLD using a multi‐atlas segmentation pipeline that allows precise measurement of MTL subregions.MethodParticipants included a diagnostically mixed sample of familial FTLD mutation carriers (MAPT: N = 87; C9orf72: N = 117; GRN: N = 65) and 142 non‐carrier family member controls from the ALLFTD study cohort (Table 1). T1‐weighted MRIs were preprocessed using a multi‐atlas automated pipeline (Automatic Segmentation of Hippocampal Subfields [ASHS]) to segment the amygdala, anterior and posterior hippocampus, and MTL cortices: entorhinal cortex (ERC), perirhinal cortex or Brodmann areas (BA) 35 and 36, and parahippocampal cortex (PHC). Following rigorous quality control and manual correction (Figure 1), we assessed bilateral volume differences in mutation carriers compared to controls, adjusted for age, sex, and total intracranial volume. Analyses were conducted separately for symptomatic (FTLD‐Clinical Dementia Rating Global Score≥0.5) and presymptomatic (Global Score = 0) mutation carriers.ResultOut of 413 scans, 317 (76.8%) segmentations passed quality control while 94 (22.8%) needed some manual edits—this was more common in symptomatic than presymptomatic carriers (32% versus 15%, p = 0.001). Three scans were excluded (<1%; 2 complete scans, 1 for selected subregions) due to insufficient landmark visibility in symptomatic GRN and MAPT carriers. In symptomatic carriers, all MTL subregions demonstrated reduced volume compared to controls, although MAPT carriers exhibited particularly marked atrophy, especially in anterior MTL subregions (Table 2; amygdala, ERC, BA35, hippocampus). Presymptomatic MAPT and C9orf72 carriers also exhibited significant atrophy of primarily anterior MTL cortices, while presymptomatic GRN carriers only exhibited significantly smaller ERC volume compared to controls.ConclusionMTL atrophy is present in genetic FTLD across mutation carriers, even at the presymptomatic stage. Anterior MTL subregions, which are also susceptible to neuropathologic accumulation in early Alzheimer’s disease and Limbic‐predominant age‐related TDP‐43 encephalopathy neuropathologic change (LATE‐NC), tend to be more vulnerable than posterior subregions. Unsurprisingly, MAPT carriers show the greatest MTL atrophy compared to controls across groups.

AbstractBackgroundApproximately 10% of patients with acute SARS‐CoV‐2 infection present with persistent symptoms recognized as the long‐COVID. Neurological and cognitive symptoms are prevalent in long‐COVID, requiring a deeper understanding of the biological basis of this condition for potential therapeutic interventions. Cerebrovascular complications are observed during acute infection, underscoring the importance of understanding cerebrovascular outcomes. Our objective was to investigate white matter hyperintensities (WMH) as a potential indicator of microvascular disease associated with long‐COVID in the brainMethodParticipants with long‐COVID (n = 71; hospitalized,n = 9,non‐hospitalized,n = 62 during the acute infection) and controls(n = 50) who did not have a reported infection were recruited from the community at five US sites that participated in the COVID‐Brain‐Advanced‐Imaging‐Network (COVID‐BRAIN) project. To be included, participants with long‐COVID had to experience neurological or cognitive sequelae within 6 months after a confirmed infection and continued to show at least one neurological symptom. Clinical evaluation and MRI were performed an average of 20±10 months after the acute infection. WMH volumes were measured from the 3D T2‐weighted FLAIR MRIs using a semi‐automated approach for the WMH segmentation. A linear regression model predicting log transformed WMH volume was used to investigate the association of age, groups (hospitalized long‐COVID, non‐hospitalized long‐COVID, controls), and the interaction of these two predictors along with an adjustment for total intracranial volume. Additionally, predicted regression lines have been plotted on the untransformed WMH scale.ResultWe observed a greater age‐dependent increase in WMH volumes in the hospitalized patients with long‐COVID compared to the control group (group*age interaction, p = 0.005). We did not observe this difference in slopes in the non‐hospitalized patients with long‐COVID compared to the controls. Figure shows the slopes of WMH volume as a percentage of the total intracranial volume by age in the three groups.ConclusionOur preliminary observation in this ongoing long‐Covid study suggests that hospitalization during acute COVID is associated with the age‐dependent increase in microvascular injury in the white matter. Older adults with acute infection that require hospitalization are more susceptible to cerebrovascular adverse outcomes than controls or those who do not require hospitalization. Impact of such microvascular changes on cognitive outcomes and persistence of post‐COVID symptoms is yet to be studied.

AbstractBackgroundWhite matter (WM) damage is seen with neurodegenerative and cerebrovascular pathologies and contributes to cognitive dysfunction. We hypothesized that there are multiple disease progression patterns in WM microstructural changes related to aging and dementia, and these can be identified using SuStaIn (data‐driven clustering algorithm for disease subtype and stage discovery) on multishell diffusion MRI (NODDI) data. NODDI provides information on cellular tissue architecture – neurite density index (NDI) measures packing density of axons and dendrites, and free water fraction (FWF) measures unrestricted water, e.g., interstitial and cerebrospinal fluid.MethodWe identified 1136 participants (mean age=70 years, 51% male, 40% Aß‐positive, 19% MCI/AD dementia) from the Mayo ADRC and Mayo Clinic Study of Aging. We analyzed NDI and FWF in 55 WM regions. We selected a subset of WM features that monotonically changed with clinical disease progression and then applied the Z‐score SuStaIn algorithm.ResultNDI in seven regions and FWF in six regions survived selection criteria (Figure 1). Noteworthy, the NDI regions were predominantly located in the temporal lobe, while FWF regions were primarily distributed in the frontal and subcortical regions. The SuStaIn algorithm discovered two stable clusters: the NDI‐First (Temporal) and FWF‐First (Frontal) groups (Figure 2). For the NDI‐First group, temporal lobe NDI changed early followed by frontal FWF. The FWF‐First group had the reverse order. There was clear phase separation based on which measure changed first. Stages of the NDI‐First group correlated better with meta‐ROI Tau‐PET SUVR and with MMSE than the FWF‐First group (p<0.01), suggesting that NDI‐First may be indicative of a primarily AD pathway.ConclusionGiven NDI changes are likely degenerative and FWF are suggested to be other processes such as neurodegeneration or inflammatory, the observed changes in the temporal and frontal regions may be due to distinct etiologies, i.e., neurodegenerative in the temporal lobes vs. vascular dysfunction often observed in the frontal lobes. The SuStaIn algorithm showed promise in discovering disease subtypes of WM microstructural changes. Further work is needed to understand the mechanistic basis of these observed changes and integrate WM changes for differential etiology identification and disease staging.

Abstract Background Many proposed clinical decision support systems (CDSS) require multiple disparate data elements as input, which makes implementation difficult, and furthermore have a black‐box nature leading to low interpretability. Fluorodeoxyglucose Positron Emission Tomography (FDG‐PET) is an established modality for the diagnosis of dementia, and a CDSS that uses only an FDG‐PET image to produce a reliable and understandable result would ease both of these challenges to clinical application. Method A deep variational autoencoder (VAE) was used to extract a latent representation of each image through prior training from FDG‐PET brain images (n=2000). This unsupervised VAE has a novel graph convolutional architecture that makes it applicable to masked template space images. A logistic regression model was used to classify each image. A parametric study of the latent space revealed the imaging features that were used in the logistic regression model to differentiate each class. A separate dataset of participants labeled with their clinical diagnosis (n=1239) was used to assess the logistic regression model’s diagnostic accuracy of cognitively unimpaired (CU) (n=679) and between common dementia subtypes: Alzheimer’s disease (AD) (n=310), Lewy body dementia (DLB) (n=151), and behavioral variant frontotemporal dementia (bvFTD) (n=99). The logistic regression classifier was evaluated using receiver‐operator characteristic (ROC‐AUC) curves. Result ROC‐AUC curves for CU and each dementia subtype are illustrated in Figure 1a. A k nearest neighbors model was used to develop a graphical representation of the latent space where nodes are images and edges are drawn between nearest neighbors, illustrated in Figure 1b. The graph of the latent space demonstrates a comparison between dementia subtypes and CU images. Conclusion In this study, we developed a proof‐of‐concept VAE that can achieve differential diagnosis with 84.8% balanced accuracy. The model architecture provides visual interpretability, and shows that the hippocampi, parietal lobes, and cerebellum, were useful for distinguishing AD, the frontal lobe, posterior cingulate cortex, and hippocampi were useful for distinguishing DLB, and prefrontal pole, subcortical structures, and occipital lobe were useful for distinguishing bvFTD. These regions of metabolism align with clinical pathology and metabolic patterns.

AbstractBackgroundSystemic inflammation plays a pivotal role in many chronic diseases including Alzheimer’s disease (AD). Assessing the composition of immune pathways in neurodegenerative diseases can contribute to precision medicine. Using publicly available transcriptomic data, we sought to elucidate transcriptional networks pertinent to inflammatory pathways across brain regions and peripheral blood in AD/mild cognitive impairment (MCI) and peripheral blood in Parkinson’s disease (PD).MethodFor the AD/MCI vs. control dataset, we analyzed bulk‐RNAseq collected from 6 brain regions of donors from ROSMAP, Mayo Clinic, and Mount Sinai School of Medicine (MSSM) brain banks available from the AMP‐AD consortium. Ante‐mortem, blood RNAseq expression data was retrieved from the AMP‐AD Emory Vascular cohort and Mayo Clinic Study of Aging (MCSA). We also collected blood‐derived microarray expression data from the Alzheimer’s Disease Neuroimaging Initiative (ADNI). For the PD vs Control dataset, blood‐derived bulk‐RNAseq from the PDBP and PPMI cohorts were available through the AMP‐PD consortium. Following quality control, normalization, and residual generation to account for biological and technical variables, co‐expression network modules and their enriched pathways were identified using WGCNA within each dataset. Module/trait correlation tests for aging and diagnosis (cases [AD/MCI or PD] vs control) phenotypes were evaluated. Gene Ontology enrichment analyses were executed to identify enriched pathways and brain or blood cell types within the modules. Modules were tested for preservation across cohorts.ResultWe identified conserved immune signatures across brain regions and cohorts. Modules involved in immune response were preserved across all cohorts. Blood consensus modules involved in immune response were preserved in the brain and vice versa. Some immune modules were associated with AD/MCI, PD, and/or aging. Brain immune modules are significantly associated with aging and/or AD. Significant correlations (q<0.05) with PD diagnosis were present. In the MCSA and Emory vascular cohorts, there were no significant (q<0.05) associations between modules and diagnosis, while in ADNI there were nominal (p<0.05) associations.ConclusionPreserved transcriptional immune networks were identified across blood and brain and across two neurodegenerative diseases. Expanding gene co‐expression network analyses to other diseases and integrating additional omics measures and phenotypes can further strengthen these findings to unravel the immune signatures across complex diseases.

AbstractBackgroundAutosomal dominant progranulin (GRN) mutations are a common genetic cause of frontotemporal lobar degeneration. Though clinical trials for GRN‐related therapies are underway, there is an unmet need for biomarkers that can predict symptom onset and track disease progression. We previously showed that presymptomatic GRN carriers exhibit thalamocortical hyperconnectivity that increases with age when they are presumably closer to symptom onset. However, whether hyperconnectivity arises concomitantly with markers of neurodegeneration remains unclear.MethodUtilizing T1 and task‐free functional magnetic resonance imaging (tf‐fMRI) from 49 presymptomatic and 26 symptomatic GRN mutation carriers, we determined the relationships between functional connectivity as measured by voxel‐wise whole brain degree and GRN‐relevant markers of disease progression, which included plasma neurofilament light chain (NfL) concentrations, CSF complement C1q and C3b protein levels, grey matter atrophy, and OCD symptom severity.ResultNfL concentrations were associated with frontotemporoparietal and thalamic hyperconnectivity in presymptomatic GRN carriers and extensive regions of atrophy in symptomatic carriers. Complement levels were associated with regions of hyperconnectivity, but not gray matter, in symptomatic carriers. Presymptomatic carriers with thalamic hyperconnectivity tended to have lower grey matter volume in bilateral insula and left lateral parietal cortex, which are among regions that deteriorate in GRN‐FTD. OCD symptom severity was associated with hypoconnectivity across all GRN carriers.ConclusionIn presymptomatic carriers, the co‐occurrence of hyperconnectivity, high NfL, and low gray matter suggests that tf‐fMRI hyperconnectivity may portend the onset of the neurodegenerative phase. These findings point toward hyperconnectivity as an indicator of approaching symptomatic onset.

AbstractBackgroundFrontotemporal dementia (FTD) presents with heterogeneous neuropsychiatric symptoms (NPS). These symptoms often begin prior to the onset of FTD, and progress throughout the prodromal stages of FTD. Particularly, familial FTD due to autosomal dominant genetic mutations might display mutation‐specific NPS profiles. We hypothesized distinct NPS trajectories for chromosome 9 open reading frame 72 (C9orf72), progranulin (GRN), and microtubule‐associated protein tau (MAPT) mutation carriers during their transition from presymptomatic to symptomatic stages of FTD.MethodIn this study, we analyzed N = 1662 participants from the ARTFL‐LEFFTDS Longitudinal FTLD (ALLFTD) Study, with 342 C9orf72, 148 GRN, 168 MAPT mutation carriers, and 1004 noncarriers. We used the CDR plus NACC FTLD global scores to define the conversion status, and stratified participants into four stages of progression: 1) Presymptomatic (CDR = 0 throughout the follow‐up), 2) Early conversion (began with CDR = 0, then increased to 0.5), 3) Advanced conversion (began with CDR = 0.5, then increased to 1.0 or above), and 4) Symptomatic (CDR>1.0 throughout). Over up to seven visits, the Neuropsychiatric Inventory Questionnaire (NPI‐Q) assessed changes in NPS. We analyzed total NPI‐Q score trajectories using a generalized linear mixed‐effects model, adjusting for age and baseline NPI‐Q scores.ResultOur findings showed similar NPS trajectories among carriers and noncarriers during presymptomatic stages. However, in the early conversion stage, C9orf72 and GRN carriers exhibited significantly higher NPI‐Q score increases compared to MAPT carriers, primarily in the psychosis and hyperactivity domains. In the advanced and symptomatic stages, the rate of increase in NPS was not significantly different across groups.ConclusionThis study suggests that people with familial FTD, particularly those predicted to have underlying TDP‐43 pathology, may experience more severe neuropsychiatric symptoms like psychosis or hyperactivity as they progress from presymptomatic to prodromal phases. This trajectory appears distinct from those with tau pathology or sporadic FTD. Further studies are warranted to understand these unique progression patterns and their implications for FTD management.

Abstract Objective To assess the association of systolic and diastolic blood pressure (SBP and DBP) in recently menopausal women with white matter hyperintensity (WMH) volume later in life and determine whether short-term menopausal hormone therapy (mHT) modifies these associations. Methods Kronos Early Estrogen Prevention Study (KEEPS) was a multicenter, randomized, double-blinded, placebo-controlled 4-year mHT trial (oral conjugated equine estrogens or transdermal 17β-estradiol). KEEPS continuation was an observational follow-up of the participants 10 years after the end of mHT. The associations between KEEPS baseline blood pressure (BP) with KEEPS continuation WMH volume were examined adjusting for covariates in model 1 (age, total intracranial volume, study site, mHT type) and model 2 (additionally conventional CVD risk factors). Interaction terms (BP × mHT type) were added into the linear regression models. Results The mean ± SD ages of participants were 53 (±2) years at KEEPS baseline and 67 (±2) years at KEEPS continuation. Elevated BP at KEEPS baseline was associated with greater WMH volume measured 14 years later (model 1: SBP: β = 0.01 [95% CI, 0.001‐0.01] and DBP: β = 0.01 [95% CI, 0.003‐0.03]) and after additionally adjusting for CVD risk factors (model 2). We did not find any evidence that mHT versus placebo modified these associations. Topographically, higher BP was associated with greater periventricular WMH in the frontal and parietal lobes. Conclusion Our findings suggest the importance of maintaining normal BP in recently postmenopausal women with low CVD risk, irrespective of short-term mHT usage, to potentially reduce the risk of WMH later in life.