Citations 3
Application of Hi-C sequencing to detect oncogene rearrangements for diagnosis and treatment of large B-cell lymphoma
Wang, Chang , 吴得红, Tzankov, Alexandar, Møller, Michael , Visco, Carlo , Hsi, Eric D. , Zhao, Xiaoxian , Lv, Beibei, Wang, Bangchen , Yao, Shuna, Chiu, April , Dybkær, Karen , Bhagat, Govind , Montes-Moreno, Santiago, Pan, Zenggang , Bernal‐Mizrachi, Leon , Krieken, J Han JM van, Choi, William W.L., Tam, Wayne , Chen, Weina , Ponzoni, Maurilio, Ferreri, Andrés J.M. , Khajouei, Farzaneh , Sikkink, Kristin , Schmitt, Anthony D. , Xu-Monette, Zijun Y , Møller, Michael Boe , Visco, Carlo , Hsi, Eric D , Lv, Beibei , Wang, Bangchen , Chiu, April , Dybkaer, Karen , Bhagat, Govind , Chen, Weina , Parsons, Benjamin M , Khajouei, Farzaneh , Sikkink, Kristin , Li, Yong , Young, Ken H , Xu‐Monette, Zijun Y. , Tzankov, Alexandar , Cao, Xin , Zu, Youli , Montes‐Moreno, Santiago , Krieken, J. Han van , Ponzoni, Maurilio , Parsons, Benjamin M. , Li, Yong , Hastie, Alex R. , Young, Ken H.
Blood Advances | June 17, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
10.1182/bloodadvances.2026019675
Diffuse large B-cell lymphoma (DLBCL), the most common type of lymphoma, arises from various pathogenic mechanisms including gene translocations and fusions. Detection of gene rearrangements using fluorescence in situ hybridization (FISH) is a standard practice for DLBCL diagnosis and guides treatment decisions. High-throughput chromosome conformation capture (Hi-C) DNA sequencing is a next-generation sequencing-based technology to identify genome-wide rearrangements using a single assay. In this study, Hi-C sequencing was performed using FFPE tissues from 159 patients with DLBCL, and identified 746 cancer genes at or proximal to the rearrangement breakpoints with a total of 1903 occurrences. Focusing on clinically important rearrangements, Hi-C detected 102 rearrangements of MYC, BCL2, and/or BCL6 in 92 patients and revealed the fusion partners, including 25 rearrangements missed by FISH. Causes of FISH negative results included FISH-cryptic breakpoints, complex or faint FISH signals, low percentage of FISH positivity, and issues related to tissue fixation. Moreover, in 20 patients (12.6%), Hi-C sequencing detected 22 rearrangements characteristic of other lymphoma types, including CCND1 rearrangements that could lead to reclassification as mantle cell lymphoma. Survival analysis for genome-wide rearrangements using machine learning models identified MYC, PD-L1, CCND1, BCL2, NTRK1/PRCC, RRAS, and FANCE rearrangements with significant prognostic effects in the DLBCL cohort. In conclusion, Hi-C sequencing detects gene rearrangements crucial for diagnosis in an unbiased and molecular manner and showed high sensitivity and specificity in our study. These advantages of Hi-C sequencing offer help to improve the workflow of clinical pathology laboratories, diagnostic precision, and treatment of large B-cell lymphoma.
Functions of flt3 in zebrafish hematopoiesis and its relevance to human acute myeloid leukemia
He, Bai‐Liang , Shi, Xiangguo , Man, Cheuk Him , C.H., Alvin, Ekker, Stephen C. , Chow, Howard C.H., So, Chi Wai Eric , Choi, William W.L., Leung, Anskar Y.H. , Zhang, Wenqing , Zhang, Yiyue , Alvin, C. H. , Ekker, Stephen C
Blood | March 4, 2014 | Volume: 123 | Issue: 16 | Pages: 2518-2529
Key Points Zebrafish flt3 plays an important role in the initiation of definitive hematopoietic stem cells. Expression of human FLT3-ITD activates endogenous flt3 signaling and induces myeloid expansion.
MDM2 phenotypic and genotypic profiling, respective to TP53 genetic status, in diffuse large B-cell lymphoma patients treated with rituximab-CHOP immunochemotherapy: a report from the International DLBCL Rituximab-CHOP Consortium Program
Xu‐Monette, Zijun Y. , Møller, Michael , Tzankov, Alexandar , Montes‐Moreno, Santiago , Hu, Wenwei , Manyam, Ganiraju C. , Kristensen, Louise , Fan, Lei , Visco, Carlo , Dybkær, Karen , Chiu, April , Tam, Wayne , Zu, Youli , Richards, Kristy L. , Choi, William W.L., Krieken, J. Han van , Huang, Qin , Huh, Jooryung , Ferreri, Andrés J.M. , Zhao, Xiaoying , Bueso‐Ramos, Carlos E. , Wang, Sa A. , Winter, Jane N. , Piris, Miguel Á. , Medeiros, L. Jeffrey , Young, Ken H. , Bhagat, Govind , Hsi, Eric D. , Ai, Weiyun Z. , Ponzoni, Maurilio , Wu, Lin , Go, Ronald S. , Li, Yong , Møller, Michael Boe , Winter, Jane N , Tzankov, Alexandar , Dybkaer, Karen , van Krieken, J Han , Young, Ken H , Piris, Miguel , Medeiros, L Jeffrey , Zu, Youli , Montes-Moreno, Santiago , Xu-Monette, Zijun Y
Blood | August 28, 2013 | Volume: 122 | Issue: 15 | Pages: 2630-2640
Key PointsPhenotypic and genotypic profiling of MDM2 in DLBCL. MDM2 as a negative regulator of p53 tumor suppressor function.