Chen, Junyu
Mayo Clinic
State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Citations 2
The Proposed Bone Post-Arterial Type R Capillaries Resolve into Venous and Fatty Acid-Handling Endothelial Cells
Jain, Sanyam , Joseph, Jessy Davina, Liu, Hanyu , Bhowmick, Nainita, Yang, Yang, Mundapat, Siddharth Nishant, Glaviano, Antonino , Chandra, Abhishek , Dalan, Rinkoo , Chen, Junyu , Risbud, Makarand V. , Bözec, Aline , Roy, Nainita , Machan, Radek , Chandra, Abhishek , Dalan, Rinkoo , Chen, Junyu , Verma, Navin Kumar , Cohen-Solal, Martine , Risbud, Makarand V , Bozec, Aline , Kusumbe, Anjali P , Joseph, Jessy D
No container title available | July 28, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
Abstract Endothelial specialization is increasingly recognized as a fundamental regulator of tissue homeostasis, yet the cellular diversity of the skeletal vasculature remains incompletely resolved. Here, we integrate large-scale single-cell transcriptomics, cross-tissue comparisons, and imaging to comprehensively define endothelial heterogeneity across the skeleton. Our analyses demonstrate that the proposed post- arterial “type R” endothelial population is not a distinct endothelial subtype but instead comprises canonical venous endothelial cells and fatty acid-handling endothelial state. RNA velocity supports a venous continuum, while the proposed type R markers FMO2, and AQP7 lack both endothelial and skeletal specificity. The fatty acid-handling endothelial state, characterized by Lpl and Cd36 is conserved across multiple skeletal sites and non-skeletal tissues, indicating a general endothelial metabolic programme. Within bone, this endothelial state expands following high-fat diet and is suppressed during injury. Together, these findings redefine skeletal endothelial heterogeneity and establish the proposed type R population as part of a venous continuum.
Protocol for standardized minimally invasive mouse models of bisphosphonate-related and radiation-induced jaw osteonecrosis
Ding, Zhangfan , Risbud, Makarand V. , Chen, Junyu , Kusumbe, Anjali P , Chen, Junyu , 张家源 , Liu, Hanyu , Chandra, Abhishek
No container title available | July 3, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
SUMMARY This protocol describes a standardized and reproducible minimally invasive approach for establishing mouse models of bisphosphonate-related osteonecrosis of the jaw (BRONJ) and osteoradionecrosis of the jaw (ORNJ). The method combines a unified low-trauma oral surgical procedure with disease-specific injury induction strategies to generate robust and clinically relevant models of jaw osteonecrosis. For BRONJ, systemic zoledronic acid administration is coupled with mandibular first molar extraction using tape-assisted mouth opening and customized bent micro-forceps, minimizing soft tissue damage and reducing procedural variability. For ORNJ, a customized lead-shielding platform enables precise, noninvasive mandible-targeted irradiation, producing reproducible bone injury while limiting off-target radiation exposure. Together, these complementary models provide a consistent and minimally invasive framework for investigating jaw osteonecrosis arising from distinct etiologies. The protocol supports comprehensive downstream analyses, including micro-computed tomography, histology, and immunofluorescence, and facilitates mechanistic studies of disease pathogenesis, bone regeneration, and therapeutic intervention.