Citations 1
The Proposed Bone Post-Arterial Type R Capillaries Resolve into Venous and Fatty Acid-Handling Endothelial Cells
Jain, Sanyam , Joseph, Jessy Davina, Liu, Hanyu , Bhowmick, Nainita, Yang, Yang, Mundapat, Siddharth Nishant, Glaviano, Antonino , Chandra, Abhishek , Dalan, Rinkoo , Chen, Junyu , Risbud, Makarand V. , Bözec, Aline , Roy, Nainita , Machan, Radek , Chandra, Abhishek , Dalan, Rinkoo , Chen, Junyu , Verma, Navin Kumar , Cohen-Solal, Martine , Risbud, Makarand V , Bozec, Aline , Kusumbe, Anjali P , Joseph, Jessy D
No container title available | July 28, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
Abstract Endothelial specialization is increasingly recognized as a fundamental regulator of tissue homeostasis, yet the cellular diversity of the skeletal vasculature remains incompletely resolved. Here, we integrate large-scale single-cell transcriptomics, cross-tissue comparisons, and imaging to comprehensively define endothelial heterogeneity across the skeleton. Our analyses demonstrate that the proposed post- arterial “type R” endothelial population is not a distinct endothelial subtype but instead comprises canonical venous endothelial cells and fatty acid-handling endothelial state. RNA velocity supports a venous continuum, while the proposed type R markers FMO2, and AQP7 lack both endothelial and skeletal specificity. The fatty acid-handling endothelial state, characterized by Lpl and Cd36 is conserved across multiple skeletal sites and non-skeletal tissues, indicating a general endothelial metabolic programme. Within bone, this endothelial state expands following high-fat diet and is suppressed during injury. Together, these findings redefine skeletal endothelial heterogeneity and establish the proposed type R population as part of a venous continuum.