Citations 2
The Proposed Bone Post-Arterial Type R Capillaries Resolve into Venous and Fatty Acid-Handling Endothelial Cells
Jain, Sanyam , Joseph, Jessy Davina, Liu, Hanyu , Bhowmick, Nainita, Yang, Yang, Mundapat, Siddharth Nishant, Glaviano, Antonino , Chandra, Abhishek , Dalan, Rinkoo , Chen, Junyu , Risbud, Makarand V. , Bözec, Aline , Roy, Nainita , Machan, Radek , Chandra, Abhishek , Dalan, Rinkoo , Chen, Junyu , Verma, Navin Kumar , Cohen-Solal, Martine , Risbud, Makarand V , Bozec, Aline , Kusumbe, Anjali P , Joseph, Jessy D
No container title available | July 28, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
Abstract Endothelial specialization is increasingly recognized as a fundamental regulator of tissue homeostasis, yet the cellular diversity of the skeletal vasculature remains incompletely resolved. Here, we integrate large-scale single-cell transcriptomics, cross-tissue comparisons, and imaging to comprehensively define endothelial heterogeneity across the skeleton. Our analyses demonstrate that the proposed post- arterial “type R” endothelial population is not a distinct endothelial subtype but instead comprises canonical venous endothelial cells and fatty acid-handling endothelial state. RNA velocity supports a venous continuum, while the proposed type R markers FMO2, and AQP7 lack both endothelial and skeletal specificity. The fatty acid-handling endothelial state, characterized by Lpl and Cd36 is conserved across multiple skeletal sites and non-skeletal tissues, indicating a general endothelial metabolic programme. Within bone, this endothelial state expands following high-fat diet and is suppressed during injury. Together, these findings redefine skeletal endothelial heterogeneity and establish the proposed type R population as part of a venous continuum.
Protocol for standardized minimally invasive mouse models of bisphosphonate-related and radiation-induced jaw osteonecrosis
Ding, Zhangfan , Risbud, Makarand V. , Chen, Junyu , Kusumbe, Anjali P , Chen, Junyu , 张家源 , Liu, Hanyu , Chandra, Abhishek
No container title available | July 3, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
SUMMARY This protocol describes a standardized and reproducible minimally invasive approach for establishing mouse models of bisphosphonate-related osteonecrosis of the jaw (BRONJ) and osteoradionecrosis of the jaw (ORNJ). The method combines a unified low-trauma oral surgical procedure with disease-specific injury induction strategies to generate robust and clinically relevant models of jaw osteonecrosis. For BRONJ, systemic zoledronic acid administration is coupled with mandibular first molar extraction using tape-assisted mouth opening and customized bent micro-forceps, minimizing soft tissue damage and reducing procedural variability. For ORNJ, a customized lead-shielding platform enables precise, noninvasive mandible-targeted irradiation, producing reproducible bone injury while limiting off-target radiation exposure. Together, these complementary models provide a consistent and minimally invasive framework for investigating jaw osteonecrosis arising from distinct etiologies. The protocol supports comprehensive downstream analyses, including micro-computed tomography, histology, and immunofluorescence, and facilitates mechanistic studies of disease pathogenesis, bone regeneration, and therapeutic intervention.