Citations 2
Evaluation of LightCycler PCR for Implementation of Laboratory Diagnosis of Herpes Simplex Virus Infections
Espy, Mark J., Ross, Teri K., Teo, Rosaline, Svien, Kathleen A., Wold, Arlo D., Uhl, James R., Smith, Thomas F.
Journal of Clinical Microbiology | December 31, 2019 | Volume: 38 | Issue: 8 | Pages: 3116-3118
10.1128/jcm.38.8.3116-3118.2000
ABSTRACT Five hundred specimens (288 genital, 192 dermal, and 20 ocular) were extracted by technologists, and the DNA was assayed by LightCycler PCR (DNA polymerase and thymidine kinase [TK] gene targets) and by conventional tube and shell vial cell culture. One hundred fifty-eight confirmed (by cell culture and TK target PCR) positive and LightCycler-positive specimens were detected during the first 30 PCR cycles. LightCycler PCR-positive results for cycles 31 to 45 (39 of 67 [58.2%]) required confirmation by another PCR target (TK). LightCycler PCR is more sensitive ( n = 197; 23.1%) than cell cultures ( n = 150) for the routine laboratory detection of herpes simplex virus infections.
Seasonality of Bartonella henselae IgM and IgG Antibody Positivity Rates
Theel, Elitza S. , Ross, Teri K., Ross, Teri
Journal of Clinical Microbiology | September 16, 2019 | Volume: 57 | Issue: 12 | Pages: No page available