Citations 2
Evaluation of the NG-Test MCR-1 Lateral Flow Assay and EDTA-Colistin Broth Disk Elution Methods To Detect Plasmid-Mediated Colistin Resistance among Gram-Negative Bacterial Isolates
Fenwick, Alexander J. , Bergman, Yehudit , Lewis, Shawna, Yee, Rebecca , Cole, Nicolynn C., Kohner, Peggy C., Ordak, Carly, Green, Daniel A. , Humphries, Romney M. , Simner, Patricia J. , Cole, Nicolynn, Kohner, Peggy, Green, Daniel A , Humphries, Romney , Uhlemann, Anne‐Catrin , Schuetz, Audrey N. , Bergman, Yehudit
Journal of Clinical Microbiology | January 24, 2020 | Volume: 58 | Issue: 4 | Pages: No page available
Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-colistin broth disk elution (EDTA-CBDE) screening test method. These methods were evaluated using a cohort of contemporary, clinical Gram-negative bacillus isolates from 3 U.S. academic medical centers (126 isolates of the Enterobacterales , 50 Pseudomonas aeruginosa isolates, and 50 Acinetobacter species isolates; 1 isolate was mcr positive) and 12 mcr -positive CDC-FDA Antibiotic Resistance (AR) Isolate Bank isolates for which reference broth microdilution colistin susceptibility results were available.
Multicenter Evaluation of Colistin Broth Disk Elution and Colistin Agar Test: a Report from the Clinical and Laboratory Standards Institute
Humphries, Romney M. , Green, Daniel A. , Bergman, Yehudit , Lewis, Shawna, Yee, Rebecca , Stump, Stephania , Lopez, Mabel, Maćešić, Nenad , Uhlemann, Anne‐Catrin , Kohner, Peggy C., Cole, Nicolynn C., Simner, Patricia J. , Uhlemann, Anne-Catrin , Kohner, Peggy, Cole, Nicolynn, Humphries, Romney , Schuetz, Audrey N. , Lopez, Mabel, Bergman, Yehudit
Journal of Clinical Microbiology | September 10, 2019 | Volume: 57 | Issue: 11 | Pages: No page available
Susceptibility testing of the polymyxins (colistin and polymyxin B) is challenging for clinical laboratories. The Clinical and Laboratory Standards Institute (CLSI) Antimicrobial Susceptibility Testing Subcommittee evaluated two methods to enable accurate testing of these agents. These methods were a colistin broth disk elution (CBDE) and a colistin agar test (CAT), the latter of which was evaluated using two inoculum volumes, 1 μl (CAT-1) and 10 μl (CAT-10).