Citations 1
Evaluation of the NG-Test MCR-1 Lateral Flow Assay and EDTA-Colistin Broth Disk Elution Methods To Detect Plasmid-Mediated Colistin Resistance among Gram-Negative Bacterial Isolates
Fenwick, Alexander J. , Bergman, Yehudit , Lewis, Shawna, Yee, Rebecca , Cole, Nicolynn C., Kohner, Peggy C., Ordak, Carly, Green, Daniel A. , Humphries, Romney M. , Simner, Patricia J. , Cole, Nicolynn, Kohner, Peggy, Green, Daniel A , Humphries, Romney , Uhlemann, Anne‐Catrin , Schuetz, Audrey N. , Bergman, Yehudit
Journal of Clinical Microbiology | January 24, 2020 | Volume: 58 | Issue: 4 | Pages: No page available
Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-colistin broth disk elution (EDTA-CBDE) screening test method. These methods were evaluated using a cohort of contemporary, clinical Gram-negative bacillus isolates from 3 U.S. academic medical centers (126 isolates of the Enterobacterales , 50 Pseudomonas aeruginosa isolates, and 50 Acinetobacter species isolates; 1 isolate was mcr positive) and 12 mcr -positive CDC-FDA Antibiotic Resistance (AR) Isolate Bank isolates for which reference broth microdilution colistin susceptibility results were available.