Stump, Stephania
https://orcid.org/0000-0002-8319-3893
Mayo Clinic
Columbia University Irving Medical Center
Citations 2
Evaluation of Calcium-Enhanced Media for Colistin Susceptibility Testing by Gradient Agar Diffusion and Broth Microdilution
Green, Daniel A. , Maćešić, Nenad , Lopez, Mabel, Stump, Stephania , Whittier, Susan , Simner, Patricia J. , Humphries, Romney M. , Humphries, Romney , Uhlemann, Anne‐Catrin , Lopez, Mabel, Schuetz, Audrey N.
Journal of Clinical Microbiology | November 20, 2019 | Volume: 58 | Issue: 2 | Pages: No page available
Despite the increasing reliance on polymyxin antibiotics (polymyxin B and colistin) for treatment of multidrug-resistant Gram-negative infections, many clinical laboratories are unable to perform susceptibility testing due to the lack of accurate and reliable methods. Although gradient agar diffusion is commonly performed for other antimicrobials, its use for polymyxins is discouraged due to poor performance characteristics.
Multicenter Evaluation of Colistin Broth Disk Elution and Colistin Agar Test: a Report from the Clinical and Laboratory Standards Institute
Humphries, Romney M. , Green, Daniel A. , Bergman, Yehudit , Lewis, Shawna, Yee, Rebecca , Stump, Stephania , Lopez, Mabel, Maćešić, Nenad , Uhlemann, Anne‐Catrin , Kohner, Peggy C., Cole, Nicolynn C., Simner, Patricia J. , Uhlemann, Anne-Catrin , Kohner, Peggy, Cole, Nicolynn, Humphries, Romney , Schuetz, Audrey N. , Lopez, Mabel, Bergman, Yehudit
Journal of Clinical Microbiology | September 10, 2019 | Volume: 57 | Issue: 11 | Pages: No page available
Susceptibility testing of the polymyxins (colistin and polymyxin B) is challenging for clinical laboratories. The Clinical and Laboratory Standards Institute (CLSI) Antimicrobial Susceptibility Testing Subcommittee evaluated two methods to enable accurate testing of these agents. These methods were a colistin broth disk elution (CBDE) and a colistin agar test (CAT), the latter of which was evaluated using two inoculum volumes, 1 μl (CAT-1) and 10 μl (CAT-10).