Citations 2
Ablation of immunity to Amblyomma americanum by anti-basophil serum: cooperation between basophils and eosinophils in expression of immunity to ectoparasites (ticks) in guinea pigs.
Galli, SJ, Gleich, Gerald J. , Askenase, Philip W., Brown, S J
The Journal of Immunology | January 2, 2023 | Volume: 129 | Issue: 2 | Pages: 790-796
Abstract Basophils infiltrate the skin and other tissues as part of T lymphocyte and/or antibody-mediated immune responses to certain protein antigens, viruses, tumors, and parasites. Although basophils may comprise a significant fraction of leukocytes in these reactions, their precise role has been poorly understood. Guinea pigs expressing acquired immunity to the tick Amblyomma americanum develop basophil- and eosinophil-rich cutaneous inflammatory responses at tick feeding sites, and tick rejection is associated with extensive local basophil degranulation. We report that a specific anti-basophil serum (ABS) eliminate basophils at tick feeding sites and abolished immunity to A. americanum. ABS does not react with eosinophils and did not reduce blood or bone marrow eosinophils; however, ABS-treated animals had diminished eosinophils at tick feeding sites, perhaps because these sites were deficient in basophil-derived eosinophil-chemotactic factors. AES treatment markedly reduced feeding site eosinophils, did not affect basophil levels, and partially impaired tick resistance. Taken together, our experiments suggest cooperation between basophils and eosinophils in the expression of immunity to ticks in guinea pigs.
A novel community driven software for functional enrichment analysis of extracellular vesicles data
Pathan, Mohashin , Keerthikumar, Shivakumar , Chisanga, David , Ang, Ching‐Seng , Askenase, Philip W., Batagov, Arsen O., Benito‐Martín, Alberto , Clayton, Aled , Collino, Federica , Vizio, Dolores Di , Falcón‐Pérez, Juan Manuel , Fonseca, Pedro , Fonseka, Pamali , Fontana, Simona , Gho, Yong Song , Hendrix, An , Hoen, Esther N. M. Nolte-â€TMt, Iraci, Nunzio , Kastaniegaard, Kenneth , Kislinger, Thomas , Kowal, Joanna , Kurochkin, Igor V. , Leonardi, Tommaso , Liang, Yaxuan , Llorente, Alicia , Lunavat, Taral R. , Maji, Sayantan , Øverbye, Anders , Peinado, Héctor , Principe, Simona, Ronquist, G. , Royo, Félix , Sahoo, Susmita , Spinelli, Cristiana , Stensballe, Allan , Théry, Clotilde , Herwijnen, Martijn J. C. van , Wauben, Marca H. M. , Welton, Joanne L. , Zhao, Kening , Mathivanan, Suresh , Fonseca, Pedro , Fonseka, Pamali , Fontana, Simona , Iraci, Nunzio , Kastaniegaard, Kenneth , Kowal, Joanna , Leonardi, Tommaso , Øverbye, Anders , Peinado, Héctor , Spinelli, Cristiana , Welton, Joanne L , Alessandro, Riccardo , Camussi, Giovanni , Hoen, Esther N. M. Nolte‐‘t , Villafiorita‐Monteleone, Francesca , Panaretakis, Theocharis , Patel, Tushar , Pluchino, Stefano , Falcón-Perez, Juan Manual
Journal of Extracellular Vesicles | May 26, 2017 | Volume: 6 | Issue: 1 | Pages: No page available
ABSTRACTBioinformatics tools are imperative for the in depth analysis of heterogeneous high‐throughput data. Most of the software tools are developed by specific laboratories or groups or companies wherein they are designed to perform the required analysis for the group. However, such software tools may fail to capture “what the community needs in a tool”. Here, we describe a novel community‐driven approach to build a comprehensive functional enrichment analysis tool. Using the existing FunRich tool as a template, we invited researchers to request additional features and/or changes. Remarkably, with the enthusiastic participation of the community, we were able to implement 90% of the requested features. FunRich enables plugin for extracellular vesicles wherein users can download and analyse data from Vesiclepedia database. By involving researchers early through community needs software development, we believe that comprehensive analysis tools can be developed in various scientific disciplines.