Citations 1
The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants.
Landias, D, Beck, Barbara, Buerstedde, J M, Degraw, S, Koch, N., Pierres, Michel, Tada, Tomohiro , Benoist, C, Mathis, D, Klein, David C. , Murphy, David , Yamamoto, Keiko , Benoist, Christophe O. , Mathis, Diane J.
The Journal of Immunology | December 31, 2022 | Volume: 137 | Issue: 9 | Pages: 3002-3005
Abstract The chain specificities of 18 Ak and 26 Ab-reactive anti-Ia monoclonal antibodies have been determined. L cells were transfected with haplotype-matched (A alpha k:A beta k, A alpha b:A beta b) or haplotype-mismatched (A alpha k:A beta b, A alpha b:A beta k) cDNA pairs, lines expressing high levels of surface A complex were selected, and antibody reactivity with a panel of reagents was assessed by cytofluorimetric analysis. Most of the antibodies recognized a determinant specified by one chain, either alpha or (more commonly) beta. A few examples of more complex determinants were also observed. A knowledge of the chain specificities of anti-Ia monoclonal antibodies should prove useful for a variety of studies aimed at dissecting Ia structure-function relationships.