Citations 2
The structure-function relationship of I-A molecules: correlation of serologic and functional phenotypes of four I-Ak mutant cell lines.
Beck, Barbara, Glimcher, Laurie H. , Nilson, A, Pierres, Michel, McKean, D J, Beck, B N
The Journal of Immunology | January 1, 2023 | Volume: 133 | Issue: 6 | Pages: 3176-3182
Abstract We have isolated and characterized four mutant I-Ak-expressing cell lines derived from the B cell-B lymphoma hybrid antigen-presenting cell line TA3. The mutants were isolated by first selecting against expression of one Ak epitope by treatment with a monoclonal antibody in the presence of complement and then selecting for retention of a second Ak epitope by electronic cell-sorting of cells stained for fluorescence with a second monoclonal antibody. The serologic and functional phenotypes of the mutants were characterized by using panels of I-Ak-specific monoclonal antibodies and I-Ak-restricted T hybridomas. We obtained one Ak alpha mutant (J4) that no longer reacts with any Ak alpha-specific antibody and also is incapable of stimulating any I-Ak-restricted T hybridoma. We obtained three Ak beta mutants (LD3, K5, G1) that express a wide range of serologic and functional phenotypes. Correlation of the serologic and functional phenotypes reveals that the serologic epitope Ia.1 may overlap with a major site of T cell recognition, whereas the Ia.17 serologic epitope appears to be only a minor site for T cell recognition.
The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants.
Landias, D, Beck, Barbara, Buerstedde, J M, Degraw, S, Koch, N., Pierres, Michel, Tada, Tomohiro , Benoist, C, Mathis, D, Klein, David C. , Murphy, David , Yamamoto, Keiko , Benoist, Christophe O. , Mathis, Diane J.
The Journal of Immunology | December 31, 2022 | Volume: 137 | Issue: 9 | Pages: 3002-3005
Abstract The chain specificities of 18 Ak and 26 Ab-reactive anti-Ia monoclonal antibodies have been determined. L cells were transfected with haplotype-matched (A alpha k:A beta k, A alpha b:A beta b) or haplotype-mismatched (A alpha k:A beta b, A alpha b:A beta k) cDNA pairs, lines expressing high levels of surface A complex were selected, and antibody reactivity with a panel of reagents was assessed by cytofluorimetric analysis. Most of the antibodies recognized a determinant specified by one chain, either alpha or (more commonly) beta. A few examples of more complex determinants were also observed. A knowledge of the chain specificities of anti-Ia monoclonal antibodies should prove useful for a variety of studies aimed at dissecting Ia structure-function relationships.