Citations 2
Extended normothermic machine perfusion preserves viability and tissue integrity of ex vivo human intestine segments
Urbina, Pamela A. Manco, Choi, Sae Rome, Cheung, Dillon C., Egbert, Lena K. , Garcia-Neuer, Marlene I., Yu, Stephanie, Rehman, Nisha S., Hanks, Danielle G., Le, Tuan‐Anh , Alabay, Husnu H., McHugh, Kelsey , Hamdan, Feda H., Naik, Nimesh, Faubion, William A. , Brady, Justin T. , Ceylan, Hakan , Bose, Suman , Urbina, Pamela Allison Manco , Garcia-Neuer, Marlene , Rehman, Nabeela, Alabay, Husnu Halid , Hamdan, Feda H. , Naik, Nimesh D.
No container title available | September 18, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
ABSTRACT Human intestinal diseases, including inflammatory bowel disease (IBD), are difficult to model because existing animal and in vitro systems do not capture the intestine’s integrated vascular, immune, and metabolic complexity. Here, we establish a custom normothermic machine perfusion platform that maintains surgically resected human intestinal tissue for up to 72 hours under near-physiological conditions. Discarded specimens from patients with nonmalignant intestinal diseases, including IBD and diverticulitis, were continuously perfused at 37°C with real-time hemodynamic and metabolic monitoring. Perfusion preserved tissue viability, mucosal architecture, epithelial integrity, oxygen consumption, metabolic activity, and vascular patency. Daily perfusate sampling enabled longitudinal profiling and experimental modulation of inflammatory mediators, revealing patient-specific immune states. This long-duration platform bridges reductionist in vitro models and clinical disease, enabling mechanistic studies of intestinal inflammation and future testing of personalized therapies in intact patient-derived tissue.
Endovascular treatment of distal thoracic aortic transection associated with severe thoracolumbar spinal fracture
Chock, Megan M. , Aho, Johnathon M. , Naik, Nimesh D. , Heller, Stephanie F. , Aho, Johnathon, Naik, Nimesh, Clarke, Michelle, Heller, Stephanie, Clarke, Michelle J. , Oderich, Gustavo S.
Vascular | November 18, 2014 | Volume: 23 | Issue: 5 | Pages: 550-552
Endovascular repair has become the first line of treatment in most patients with blunt aortic injury. The most common mechanism is deceleration injury affecting the aortic isthmus distal to the origin of the left subclavian artery. Injuries of the distal thoracic aorta are uncommon. We report the case of a 25-year-old male patient who presented with paraplegia and distal thoracic aortic pseudoaneurysm associated with severe thoracolumbar vertebral fracture and displacement after a motocross accident. Endovascular repair was performed using total percutaneous technique and conformable C-TAG thoracic stent-graft (WL Gore, Flagstaff, AZ). Following stent-graft placement and angiographic confirmation of absence of endoleak, thoracolumbar spinal fixation was performed in the same operative procedure. This case illustrates a multispecialty approach to complex aortic and vertebral injury and the high conformability of newer thoracic stent-grafts to adapt to tortuous anatomy.