Citations 1
Enhanced detection of dermal-binding autoantibodies in pemphigoid diseases with a modified indirect immunofluorescence cell-based mosaic assay using IgG4 and optimized testing conditions: a single-centre retrospective study
Agrawal, Shruti , Camilleri, M. J., Comfere, Nneka I. , Johnson, Emma F. , Kalaaji, Amer N., Wieland, Carilyn N. , Lange, Colleen R., Miller, C., Shore, Carly J, Guerrico, Anatilde M Gonzalez, Lange, Colleen, Miller, Cameron, Gonzalez Guerrico, Anatilde M, Lehman, Julia S. , Guerrico, Anatilde M. Gonzalez
British Journal of Dermatology | January 9, 2026 | Volume: 195 | Issue: 1 | Pages: 187-188
Patzelt et al. previously characterized dermal-binding autoantibodies using an indirect immunofluorescence (IIF) mosaic assay with cells transfected with p200-laminin β4, laminin-332 or collagen VII subunits among specimens showing a dermal pattern on IIF using salt-split skin (IIF-SSS) and IgG. This study expanded testing to include specimens with either a dermal or mixed pattern on IIF-SSS and demonstrated improved test sensitivity with modified testing methods.