Citations 1
A brief harvesting-freezing delay significantly alters the kidney metabolome and leads to false positive and negative results
Alsawaf, Yahya , Maksimovic, Igor , Zheng, Jamie , Zhang, Song , Vučković, Ivan , Dzeja, Petras P. , Macura, Slobodan , Irazabal, María V. , Dzeja, Petras , Macura, Slobodan I , Irazabal, Maria V , Vuckovic, Ivan , Alsawaf, Yahya
American Journal of Physiology-Renal Physiology | August 29, 2024 | Volume: 327 | Issue: 5 | Pages: F697-F711
Our study shows that a brief harvesting-freezing delay associated with organ collection and freezing can significantly alter the kidney metabolic profile of both male and female wild-type and a genetic model of chronic kidney disease. Importantly, given that the effect of this delay differs among genotypes, it is not safe to assume that equally delaying harvesting-freezing in wild-type and polycystic kidney disease kidneys adequately controls this effect, ultimately leading to false positive and negative results among different renal diseases.