Citations 2
Albumin promoter-driven FlpO expression induces efficient genetic recombination in mouse liver
Zhu, Xiaohui, Yang, Yan , Feng, Dongfeng , Wang, Oliver , Chen, Jiaxiang , Wang, Jiale , Liu, Yang , Edenfield, Brandy , Haddock, Ashley , Bi, Yan , Ji, Baoan , Edenfield, Brandy H, Haddock, Ashley N, Bi, Yan , Ji, Baoan , Wang, Jiale , Zhu, Xiaohui, Wang, Oliver, Wang, Bin , Wang, Ying , Patel, Tushar
American Journal of Physiology-Gastrointestinal and Liver Physiology | March 12, 2024 | Volume: 326 | Issue: 5 | Pages: G495-G503
A liver-specific Alb-FlpO mouse line was generated. By coupling it with other existing CreERT or Cre lines, the dual recombinase approach can enable sequential gene modifications within the same cell or across various cell types in an organism for liver research through temporal and spatial gene manipulations.
A simple and effective genotyping workflow for rapid detection of CRISPR genome editing
Wang, Lingxiang, Wang, Jiale , Feng, Dongfeng , Jahan‐mihan, Yasmin , Bi, Yan , Lim, DoYoung, Ji, Baoan , Jahan-mihan, Yasmin, Bi, Yan , Ji, Baoan , Wang, Jiale , Wang, Bin , Wang, Ying
American Journal of Physiology-Gastrointestinal and Liver Physiology | February 27, 2024 | Volume: 326 | Issue: 4 | Pages: G473-G481
This study presents a streamlined, cost-effective genotyping workflow for clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) edited mouse models, focusing on trypsinogen genes. It simplifies initial F0 mouse screening using PCR and Sanger sequencing without needing exact sequences. For F1 mice, precise editing is identified through Sanger sequencing and algorithm decoding. The workflow includes a novel PCR strategy for distinguishing homozygous and heterozygous statuses in subsequent generations, effective for small deletions, multiple-gene knockouts, and knockins.