Citations 2
A spatial transcriptomics atlas of live donors reveals unique zonation patterns in the healthy human liver
Yakubovsky, Oran , Egozi, Adi, Halpern, Keren Bahar , Barkai, Tal, Harnik, Yotam , Novoselsky, Roy , Goliand, Inna , Keren‐Shaul, Hadas , Hirsch, Dana , Mayer, Chen , Pery, Ron, Pencovich, Niv , Taner, Timucin, Nachmany, Ido , Afriat, Amichay , Kohanim, Yael Korem , Golani, Ofra , Addadi, Yoseph , Kedmi, Merav , Fellus-Alyagor, Liat , Pery, Ron, Itzkovitz, Shalev
No container title available | February 23, 2025 | Volume: No volume available | Issue: No issue available | Pages: No page available
Abstract Reconstructing gene expression atlases for human tissues is challenging due to limited access to healthy samples from live donors. Neurologically deceased donors often show ischemic changes, while tissues near diseased regions may have altered gene expression. The liver, with its unique regenerative capacity, allows analysis from live healthy donors (LHDs). Using spatial transcriptomics (Visum, Visium HD and MERFISH), we analyzed 16 liver samples: eight from young LHDs and eight from patients with liver pathology, sampling ‘adjacent normal’ tissue. LHD livers displayed significant gene expression differences from ‘adjacent normal’ tissues. Hepatocytes exhibited marked zonation along the porto-central axis of liver lobules, with key functions pericentrally shifted compared to other mammals. Our atlas identified dynamic programs in early steatotic hepatocytes, showing transitions from lipid uptake in low-lipid regions to insulin hypersensitivity in high-lipid regions. This study presents a spatial gene expression reference for the healthy human liver and insights into hepatocyte adaptations in steatosis.
Perioperative Platelet Count Ratio Predicts Long-Term Survival after Left Pancreatectomy and Splenectomy for Pancreatic Adenocarcinoma
Nachmany, Ido , Meiri, Hila, Eidelman, Pavel , Ziv, Ofir, Bear, Lior, Jacoby, Harel , Eshkenazy, Rony, Pery, Ron, Pencovich, Niv , Gudmundsdottir, Hallbera , Nevo, Nadav
Journal of Clinical Medicine | February 12, 2024 | Volume: 13 | Issue: 4 | Pages: 1050
Background: The value of platelet characteristics as a prognostic factor in patients with pancreatic adenocarcinoma (PDAC) remains unclear. Methods: We assessed the prognostic ability of post-splenectomy thrombocytosis in patients who underwent left pancreatectomy for PDAC. Perioperative platelet count ratio (PPR), defined as the ratio between the maximum platelet count during the first five days following surgery and the preoperative level, was assessed in relation to long-term outcomes in patients who underwent left pancreatectomy for PDAC between November 2008 and October 2022. Results: A comparative cohort of 245 patients who underwent pancreaticoduodenectomy for PDAC was also evaluated. The median PPR among 106 patients who underwent left pancreatectomy was 1.4 (IQR1.1, 1.8). Forty-six had a PPR ≥ 1.5 (median 1.9, IQR1.7, 2.4) and 60 had a PPR < 1.5 (median 1.2, IQR1.0, 1.3). Patients with a PPR ≥ 1.5 had increased median overall survival (OS) compared to patients with a PPR < 1.5 (40 months vs. 20 months, p < 0.001). In multivariate analysis, PPR < 1.5 remained a strong predictor of worse OS (HR 2.24, p = 0.008). Among patients who underwent pancreaticoduodenectomy, the median PPR was 1.1 (IQR1.0, 1.3), which was significantly lower compared to patients who underwent left pancreatectomy (p > 0.001) and did not predict OS. Conclusion: PPR is a biomarker for OS after left pancreatectomy for PDAC. Further studies are warranted to consolidate these findings.