Citations 2
Preanalytical variables and analytes in liquid biopsy approach for brain tumors: A comprehensive review and recommendations from the RANO Group and the Brain Liquid Biopsy Consortium
Bettegowda, Chetan , Noushmehr, Houtan , Affinito, Alessandra , Ahluwalia, Manmeet S. , Ansorge, Olaf , Ayasoufi, Katayoun , Bagley, Stephen , Barnholtz‐Sloan, Jill S. , Brandsma, Dieta , Brodie, Chaya , Castro, Ana Valeria , Chang, Susan M. , Daher, Ahmad , Datta, Vineet , French, Pim J. , Golebiewska, Anna , Holdhoff, Matthias , LeBlang, Suzanne , Melin, Beatrice , Moulière, Florent , O’Leary, Claire , Robinson, Stephen , Sol, Nik , Vogelbaum, Michael A. , Walbert, Tobias , Watts, Colin , Wen, Patrick Y. , Wykes, Victoria , Yip, Stephen , Short, Susan , Barnholtz-Sloan, Jill S , Best, Myron , Brandsma, Dieta , Brüning-Richardson, Anke , Chang, Susan Marina , Condorelli, Gerolama , Daher, Ahmad , de Groot, John , French, Pim J , Galanis, Evanthia , Golebiewska, Anna , Hamerlik, Petra , Hanemann, C Oliver , Holdhoff, Matthias , Huse, Jason T. , Khasraw, Mustafa , LeBlang, Suzanne , Melin, Beatrice , Rominiyi, Ola , Roncaroli, F , Rudà, Roberta , Sol, Nik , van den Bent, Martin J , Walbert, Tobias , Watts, Colin , Weiss, Tobias , Weller, Michael , Wykes, Victoria , Yip, Stephen , Short, Susan C , Soffietti, Riccardo , Noushmehr, Houtan , Ayasoufi, Katayoun , Ahluwalia, Manmeet S , Bettegowda, Chetan , Groot, John de , Galanis, Evanthia , Hamerlik, Petra , Hanemann, C. Oliver , Khasraw, Mustafa , Rak, Janusz , Ray, Amitava, Roncaroli, Federico , Seoane, Joan , Bent, Martin J. van den , Wen, Patrick Yung , Soffietti, Riccardo
Neuro-Oncology | June 16, 2025 | Volume: 27 | Issue: 10 | Pages: 2496-2513
Abstract This review explores the pivotal role of preanalytical variables in bringing liquid biopsy approaches into the clinic for brain tumors. Preanalytical variables encompass a range of critical issues, from blood sample collection and handling to the impact of tumor heterogeneity and patient-specific factors. These variables introduce challenges such as false positives, false negatives, and variability in the analysis of tumor signals, which can hinder the diagnostic and prognostic utility of liquid biopsies. Understanding the nuances of preanalytical variables is essential for the successful implementation of liquid biopsy in clinical settings. This paper delves into strategies aimed at mitigating the influence of preanalytical variables by emphasizing the importance of standardized sample collection protocols, optimized sample processing and storage, quality control measures, and the integration of multiple liquid biopsy modalities.
Liquid biopsy in gliomas: A RANO review and proposals for clinical applications
Mellinghoff, Ingo K. , Warren, Katherine E. , Ahluwalia, Manmeet S. , Galanis, Evanthia , Jaeckle, Kurt A. , Seoane, Joan , Thon, Niklas , Umemura, Yoshie , Weller, Michael , Bent, Martin J. van den , Vogelbaum, Michael A. , Chang, Susan M. , Wen, Patrick Y. , Soffietti, Riccardo , Galanis, Evanthia , Weller, Michael , van den Bent, Martin J , Umemura, Yoshie , Ahluwalia, Manmeet S , Mellinghoff, Ingo K , Soffietti, Riccardo , Bettegowda, Chetan , Groot, John de , Gilbert, Mark R. , Rhun, Émilie Le , Rudà, Roberta , Chang, Susan Marina , Wen, Patrick Yung
Neuro-Oncology | January 5, 2022 | Volume: 24 | Issue: 6 | Pages: 855-871
Abstract Background There is an extensive literature highlighting the utility of blood-based liquid biopsies in several extracranial tumors for diagnosis and monitoring. Methods The RANO (Response Assessment in Neuro-Oncology) group developed a multidisciplinary international Task Force to review the English literature on liquid biopsy in gliomas focusing on the most frequently used techniques, that is circulating tumor DNA, circulating tumor cells, and extracellular vesicles in blood and CSF. Results ctDNA has a higher sensitivity and capacity to represent the spatial and temporal heterogeneity in comparison to circulating tumor cells. Exosomes have the advantages to cross an intact blood-brain barrier and carry also RNA, miRNA, and proteins. Several clinical applications of liquid biopsies are suggested: to establish a diagnosis when tissue is not available, monitor the residual disease after surgery, distinguish progression from pseudoprogression, and predict the outcome. Conclusions There is a need for standardization of biofluid collection, choice of an analyte, and detection strategies along with rigorous testing in future clinical trials to validate findings and enable entry into clinical practice.