Citations 2
Author response: Variations in HLA-B cell surface expression, half-life and extracellular antigen receptivity
Yarzabek, Brogan , Olson, Eli , Silva, Gayathri N. , Geretz, Aviva , Thomas, Rasmi , Krishnakumar, Sujatha , Ramón, Daniel S. , Zaitouna, Anita , Geng, Jie , Raghavan, Malini
No container title available | July 10, 2018 | Volume: No volume available | Issue: No issue available | Pages: No page available
Variations in HLA-B cell surface expression, half-life and extracellular antigen receptivity
Yarzabek, Brogan , Zaitouna, Anita , Olson, Eli , Silva, Gayathri N. , Geng, Jie , Geretz, Aviva , Thomas, Rasmi , Krishnakumar, Sujatha , Ramón, Daniel S. , Raghavan, Malini , Zaitouna, Anita J , Raghavan, Malini
eLife | July 10, 2018 | Volume: 7 | Issue: No issue available | Pages: No page available
The highly polymorphic human leukocyte antigen (HLA) class I molecules present peptide antigens to CD8+ T cells, inducing immunity against infections and cancers. Quality control mediated by peptide loading complex (PLC) components is expected to ensure the cell surface expression of stable peptide-HLA class I complexes. This is exemplified by HLA-B*08:01 in primary human lymphocytes, with both expression level and half-life at the high end of the measured HLA-B expression and stability hierarchies. Conversely, low expression on lymphocytes is measured for three HLA-B allotypes that bind peptides with proline at position 2, which are disfavored by the transporter associated with antigen processing. Surprisingly, these lymphocyte-specific expression and stability differences become reversed or altered in monocytes, which display larger intracellular pools of HLA class I than lymphocytes. Together, the findings indicate that allele and cell-dependent variations in antigen acquisition pathways influence HLA-B surface expression levels, half-lives and receptivity to exogenous antigens.