Citations 1
Unusual duplication mutation in a surface loop of human transthyretin leads to an aggressive drug-resistant amyloid disease
Klimtchuk, Elena S. , Frame, Nicholas M. , Abdullahi, Hassan, Spencer, Brian , Dasari, Surendra , Cui, Haili , Kurtin, Paul J. , Connors, Lawreen H. , Gursky, Olga , Connors, Lawreen H , Prokaeva, Tatiana B. , Berk, John L.
Proceedings of the National Academy of Sciences | June 25, 2018 | Volume: 115 | Issue: 28 | Pages: No page available
Significance We identified a one-of-a-kind duplication mutation in human transthyretin (TTR) that causes unusually aggressive systemic amyloidosis. To understand the poor response to treatment with a drug that stabilizes the TTR tetramer, we explored the structure, stability, and drug binding of recombinant proteins. The results suggested that amyloid formation could stem from global destabilization of the monomeric and tetrameric protein as well as the local disordering near the mutation site. This disordering induced proteolysis with release of aggregation-prone fragments. Alternatively, local disordering could trigger misfolding of the full-length protein by exposing an adhesive segment. Drug binding at a dimer interface distant from the mutation site did not significantly influence these pathological processes, indicating the need for alternative therapeutic targets.