Citations 1
Chloride channels in stellate cells are essential for uniquely high secretion rates in neuropeptide-stimulated Drosophila diuresis
Cabrero, Pablo , Terhzaz, Selim , Romero, Michael F. , Davies, Shireen A. , Blumenthal, Edward M. , Dow, Julian A. T. , Dow, Julian A T , Romero, Michael F
Proceedings of the National Academy of Sciences | September 17, 2014 | Volume: 111 | Issue: 39 | Pages: 14301-14306
Significance Endocrine control of chloride shunt conductance in Malpighian tubules of many insects is mediated by kinin diuretic peptides, but the route for chloride transport is unknown. We use Drosophila transgenics, combined with physiology, imaging, electrophysiology, and transgenic chloride reporter technology, to show that a chloride channel (CLC) encoded by ClC-a/CG31116 is uniquely localized to stellate cells and is essential for neuropeptide-stimulated, but not resting, levels of secretion. Therefore, we have resolved this issue in Drosophila ; kinin stimulates chloride flux by a transcellular route that is confined to the stellate cells.