Citations 1
Human Bone Marrow: A Reservoir for “Enhanced Effector Memory” CD8+ T Cells with Potent Recall Function
Zhang, Xiaoyu , Lin, Wei, Voss, Stephen G. , Hinkley, Lucinda, Westergren, Melissa, Tian, Guo‐Liang , Berry, Daniel J. , Lewellen, D. C., Vile, Richard G. , Strome, Scott E. , Dong, Haidong , Chen, Lieping , Färber, Donna L. , Berry, Daniel J
The Journal of Immunology | April 18, 2014 | Volume: 177 | Issue: 10 | Pages: 6730-6737
Abstract The role of human bone marrow (BM) CD8+ T cells in the immune response to viral Ags is poorly defined. We report here the identification and characterization of a functionally enhanced effector memory CD8+ T cell population (TEM) in the BM of patients undergoing total joint replacement for osteoarthritis. These BM-derived TEM differ strikingly from correlate cells in peripheral blood (PB), expressing elevated levels of CD27, HLA-DR, CD38, CD69, and unique patterns of chemokine receptors. Interestingly, while BM TEM have low levels of resting perforin and granzyme B, these molecules evidence profound up-regulation in response to TCR stimulation resulting in enhanced cytotoxic potential. Moreover, compared with the TEM subset in PB, BM CD8+ TEM cells demonstrate a more vigorous recall response to pooled viral Ags. Our results reveal that human BM serves as a repository for viral Ag-specific TEM with great therapeutic potential in vaccine development.