Citations 1
Systematic Bias Introduced by Genomic DNA Template Dilution in 16S rRNA Gene-Targeted Microbiota Profiling in Human Stool Homogenates
Harrington, Sean C., Jeraldo, Patricio , Johnson, Stephen , Walther-António, Marina , Multinu, Francesco , Jeraldo, Patricio R , Chia, Nicholas , Walther-Antonio, Marina R, Walther-Antonio, Marina , Chen, Jun, Chia, Nicholas
mSphere | March 13, 2018 | Volume: 3 | Issue: 2 | Pages: No page available
The genomic DNA input for stool samples utilized for microbiome composition has not been determined. In this study, we determined the reliable threshold level under which conclusions drawn from the data may be compromised. We also determined the type of microbial bias introduced by less-than-ideal genomic input.