Loh, Yuin-Han
Mayo Clinic
Epigenetics and Cell Fates Laboratory, Programme in Stem Cell, Regenerative Medicine and Aging, Institute of Molecular and Cell Biology, A*STAR, Singapore 138673, Singapore. yhloh@imcb.a-star.edu.sg zhanglf@ntu.edu.sg li.hu@mayo.edu.
Citations 2
Diversification of reprogramming trajectories revealed by parallel single-cell transcriptome and chromatin accessibility sequencing
Xing, Q. , Farran, Chadi A. El , Gautam, Pradeep , Chuah, Yu Song, Warrier, Tushar , Toh, Chi-Hang, Kang, Nam-Young , Sugii, Shigeki , Daley, George Q. , Xing, Q R , El Farran, Chadi A , Chang, Young-Tae , Xu, Jian , Collins, James J , Daley, George Q , Li, Hu , Zhang, Li-Feng , Loh, Yuin-Han , Loh, Yuin‐Han
Science Advances | September 11, 2020 | Volume: 6 | Issue: 37 | Pages: No page available
Integrative single-cell analysis de-constructs the heterogeneity of human reprogramming and uncovers its stage-specific GRNs.
Human Finger-Prick Induced Pluripotent Stem Cells Facilitate the Development of Stem Cell Banking
Tan, Hong-Kee, Toh, Cheng-Xu Delon, Ma, Dongrui , Yang, Binxia , Liu, Tongming , Lu, Jun , Wong, Chee-Wai , Tan, Tze-Kai , Syn, Christopher Kiu‐Choong , Tan, Eng-Lee, Lim, Bing , Lim, Yoon-Pin, Loh, Yuin‐Han , Li, Hu , Lim, Yoon-Pin, Cook, Stuart A. , Loh, Yuin-Han
Stem Cells Translational Medicine | March 20, 2014 | Volume: 3 | Issue: 5 | Pages: 586-598
Abstract Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients can be a good model for studying human diseases and for future therapeutic regenerative medicine. Current initiatives to establish human iPSC (hiPSC) banking face challenges in recruiting large numbers of donors with diverse diseased, genetic, and phenotypic representations. In this study, we describe the efficient derivation of transgene-free hiPSCs from human finger-prick blood. Finger-prick sample collection can be performed on a “do-it-yourself” basis by donors and sent to the hiPSC facility for reprogramming. We show that single-drop volumes of finger-prick samples are sufficient for performing cellular reprogramming, DNA sequencing, and blood serotyping in parallel. Our novel strategy has the potential to facilitate the development of large-scale hiPSC banking worldwide.