Grant, Barry J
Mayo Clinic
Division of Biological Sciences, Section of Molecular Biology, University of California, San Diego, La Jolla, CA 92093.
Citations 1
A posttranslational modification of the mitotic kinesin Eg5 that enhances its mechanochemical coupling and alters its mitotic function
Muretta, Joseph M. , Reddy, Babu J.N., Scarabelli, Guido, Thompson, Alex F. , Jariwala, Shashank , Major, Jennifer , Venere, Monica , Rich, Jeremy N. , Thomas, David D. , Stumpff, Jason , Grant, Barry J. , Gross, Steven P. , Rosenfeld, Steven S. , Grant, Barry J , Willard, Belinda B.
Proceedings of the National Academy of Sciences | February 5, 2018 | Volume: 115 | Issue: 8 | Pages: No page available
Significance Members of the kinesin superfamily serve a wide variety of functions, and a dominant narrative for these molecular motors has been that each member of the superfamily is uniquely specialized to serve a very limited set of functions. However, it is now appreciated that many members of this group serve several distinct physiological roles, and it has been unclear how these kinesins accomplish this functional flexibility. In this report, we describe a posttranslational modification of the kinesin 5 family member Eg5 that dramatically alters its chemomechanical behavior to make it function much more efficiently under load and in ensembles. This work provides the biophysical context required to mechanistically understand the effects of modified Eg5 in dividing cells.