Citations 1
TMEM119+ microglia MHC class I restricted antigen presentation impacts CD8 T cell memory, effector status, and blood-brain barrier disruption during neurotropic virus infection
Johnson, Aaron , Maynes, Mark , Thelwell, Javonte, Jin, Fang, Hansen, Michael, Jensen, Hadley, Witter, Ryann, Owens, Carley, Hassani, Asma , Lewis, Cody, Forston, Michael D. , Thelwell, Javonte S. , Jensen, Hadley E , Witter, Ryann K, Lewis, Cody L, Hassani, Asma , Seady, Marina, Maynes, Mark, Thelwell, Javonte, Jin, Fang, Witter, Ryann, Lewis, Cody
No container title available | June 24, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
Abstract The impact of microglia antigen presentation on CNS infiltrating CD8 T cells responses during neurotropic virus infection remains undefined. Using Theiler’s murine encephalomyelitis virus (TMEV) infection of neurons as a model system, we generated H-2K b and H-2D b Tmem119 conditional knockout mice. During TMEV infection, we determined using Tmem119 K b cKO mice that microglia H-2K b contributes to antiviral CD8 T cell proliferation in the brain. Meanwhile, using Tmem119 D b cKO mice we determined that microglia H-2D b increased antiviral CD8 T cell perforin levels and the capacity to induce blood-brain barrier (BBB) disruption. Furthermore, microglial H-2D b was essential for antigen-specific reactivation of CD8 tissue resident memory (TRM) cell recall responses and BBB disruption long after virus had been cleared. These findings demonstrate discrete roles of individual MHC class I genes on microglia in CD8 T cell proliferation, effector functions, memory recall and neuropathology.