Citations 2
Breast cancer ovarian metastases show increased activity of GPCR pathways
Savariau, Laura , Qin, Ye, Shah, Osama Shiraz , Basudan, Ahmed , Merkel, Chris , Sisoudiya, Saumya D. , Sivakumar, Smruthy , Sokol, Ethan , McGinn, Olivia , Li, Zheqi , Liu, Tiantong , Tasdemir, Nilgun , Tallapaneni, Pooja , Oesterreich, Steffi , Shah, Osama , Basudan, Ahmed M , Coffman, Lan , Elishaev, Esther , Lucas, Peter C , Lee, Adrian V , Oesterreich, Steffi , Li, Zheqi , Savariau, Laura , Elishaev, Esther , Atkinson, Jennifer M. , Lucas, Peter C. , Lee, Adrian V.
No container title available | August 1, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
Abstract Treatment resistance and metastases occur in 10–20% of patients with invasive lobular carcinoma (ILC), the most common special histological subtype of breast cancer. ILC metastasizes to the ovary more frequently than no special type (NST) tumors, also known as invasive ductal carcinoma (IDC). To characterize the genomic landscape of breast cancer ovarian metastases, we analyzed 15,613 local breast cancers, 22,010 non-ovarian metastases, and 246 ovarian metastases sequenced using FoundationOne®CDx or FoundationOne® assays. Ovarian metastases had enriched CDH1 , PIK3CA , and TBX3 mutations and depleted TP53 and MYC alterations relative to local breast cancers, with additional depletion of ESR1 mutations compared to non-ovarian metastases. CDH1 mutations were less frequent in ovarian metastases (47%) than local ILC (81.3%), with reduced 16q loss (64% vs 84%), indicating that ovarian metastases also arise from non-ILC tumors. We extended these findings to a UPMC cohort of 27 ovarian metastases (13 ILC, 8 IDC, 6 mixed ductal-lobular carcinoma) with patient-matched primary tumors in most cases. In both cohorts, patients with ovarian metastases were significantly younger than those with other metastatic sites. In the UPMC cohort, the most frequent mutations were in PIK3CA , CDH1 , KMT2C , FOXA1 , and RUNX1 . Transcriptomic analysis identified upregulated G protein-coupled receptor (GPCR) pathways, including metabotropic glutamate receptor signaling. Functional studies showed that calcium-sensing receptor (CaSR), a GPCR overexpressed in ovarian metastases, drives MEK/ERK-dependent migration and F-actin reorganization in ILC cell lines, enhanced by estrogen and blocked by calcilytic, MEK, or anti- estrogen treatment. Our findings inform future therapeutic targeting of ovarian metastasis.
Estrogen receptor-positive cell line xenograft models recapitulate metastatic dissemination and endocrine response of invasive lobular breast carcinoma
Tasdemir, Nilgun , Scott, Julie A., Biery, Kyle, Li, Zheqi , Bossart, Emily A., Wang, Sarah , Nasrazadani, Azadeh , Qin, Ye Jun, Cao, Ye , Castro, Carlos A. , Davidson, Nancy E. , Oesterreich, Steffi, Li, Zheqi , Tseng, George , Hooda, Jagmohan , Lee, Adrian V , Savariau, Laura , Latoche, Joseph D. , Sreekumar, Sreeja , Brown, Daniel D. , Watters, Rebecca Jean , Chen, Fangyuan , Sikora, Matthew J. , Anderson, Carolyn J. , Atkinson, Jennifer M. , Lucas, Peter C. , Lee, Adrian V. , Oesterreich, Steffi
No container title available | March 18, 2026 | Volume: No volume available | Issue: No issue available | Pages: No page available
Invasive lobular breast carcinoma (ILC), the most common special histological subtype of breast cancer, is characterized by nearly universal expression of estrogen receptor alpha (ER) and unique sites of metastases, neither of which is fully recapitulated by genetically engineered mouse models. Using reporter-labeled ILC mouse xenografts, herein we used mammary fat pad, tail vein and intracardiac orthotopic growth to analyze spontaneous and experimental metastasis and gene expression. We observed ER-positive primary tumors with single-file histology and collagen deposition, and spontaneous metastasis from the mammary fat pad to bones, ovaries, and brain including the leptomeninges, thereby closely mirroring the growth and metastatic spread of human ILC. Brain metastases showed strong ER staining, confirmed by sequencing analyses which identified estrogen signaling as top activated pathway, and the lesions exhibited robust response to endocrine therapy. In summary, we report endocrine responsive mammary fat pad, tail vein and intracardiac xenografts that faithfully demonstrate unique ILC features and can serve as invaluable pre-clinical translational platforms for validating candidate ILC genetic drivers and testing novel therapeutics.