Citations 2
Inter‐operator variation in ELISPOT analysis of measles virus‐specific IFN‐γ‐secreting T cells
Ryan, Jenna E., Ovsyannikova, Inna G. , Dhiman, Neelam , Pinsky, Norman A., Vierkant, Robert A. , Jacobson, Robert M. , Poland, Gregory A., Vierkant, Robert A , Jacobson, Robert M
Scandinavian Journal of Clinical and Laboratory Investigation | November 29, 2005 | Volume: 65 | Issue: 8 | Pages: 681-690
Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies
Pinsky, Norman A., Huddleston, Jeanne M. , Jacobson, Robert M. , Wollan, Peter C. , Poland, Gregory A. , Jacobson, Robert M
Clinical and Vaccine Immunology | January 9, 2003 | Volume: 10 | Issue: 1 | Pages: 19-21
ABSTRACT We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4°C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.