Tchikonia, Tamar

Aging is the leading predictor of acquired cardiovascular disease (CVD), and emerging evidence suggests cellular senescence plays a causal role in development of clinically-significant CVD. While we previously reported that senescent cell elimination delays or prevents progression of several aging phenotypes in aging mice, the responsible types of senescent cells contributing to cardiovascular dysfunction in vivo are not yet elucidated. To test the hypothesis that senescent endothelial cells are a major contributor to cardiovascular dysfunction, we developed Tie2-Cre; p16 Ink4a - LOX-ATTAC mice, which allows for specific elimination of p16 Ink4a+ senescent endothelial cells upon administration of AP20187 (which only affects cells carrying the conditionally-expressed ATTAC construct). Using a mouse model of diabetic vascular disease (40% fat diet for 12 weeks , HFD), we found that biweekly selective removal of senescent endothelial cells alleviated HFD-induced endothelial dysfunction in aorta (assessed ex vivo using isolated organ baths). Additionally, ejection fraction (assessed using in vivo echocardiography) was preserved in AP20187-treated mice (driving ATTAC-dependent removal of senescent endothelial cells) compared to their vehicle-treated controls (76.6%±4.2, 63.2%±4.2). In addition to improvement in cardiovascular function, metabolic parameters (HbA1c and fasting glucose levels) were improved by senescent endothelial cell elimination. Collectively, these data suggest that elimination of p16 Ink4a+ senescent endothelial cells may be a viable therapeutic strategy for alleviation of metabolic disease-induced cardiovascular dysfunction.