Citations 1
A mec A -Negative Strain of Methicillin-Resistant S taphylococcus aureus with Hig
Banerjee, Ritu , Gretes, Michael, Harlem, Christopher, Basuino, Li, Chambers, Henry F.
Antimicrobial Agents and Chemotherapy | August 30, 2010 | Volume: 54 | Issue: 11 | Pages: 4900-4902
ABSTRACT We previously generated a ceftobiprole-resistant S taphylococcus aureus strain after high inoculum serial passage of a mec A -negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of β-lactams but was hypersusceptible to cefoxitin.