Citations 3
A Comprehensive Approach to Sample Preparation for Electron Microscopy and the Assessment of Mitochondrial Morphology in Tissue and Cultured Cells (Adv. Biology 10/2023)
Christensen, Trace , Mungai, Margaret , Shao, Jianqiang, Trushin, Sergey , Alghanem, Ahmad F. , Jaspersen, Adam, Geroux, Rachel E., Neikirk, Kit , Biete, Michelle , Shao, Bryanna, Vue, Zer , Vang, Larry , Stephens, Dominique, Ponce, Jessica, Hicsasmaz, Innes, Edmonds, Ranthony A. C. , Dajles, Andres , Koo, Young Do , Bacevac, Serif, Salisbury, Jeffrey L., Pereira, Renata O. , Hinton, Antentor , Katti, Prasanna , Zhang, Liang , Garza-López, Edgar , Beasley, Heather K. , Marshall, Andrea G. , Stephens, Dominique C., Damo, Steven M. , Bleck, Christopher K. E. , Murray, Sandra , Glancy, Brian , Trushina, Eugenia , Abel, E. Dale
Advanced Biology | October 23, 2023 | Volume: 7 | Issue: 10 | Pages: No page available
Three-dimensional mitochondria reconstructions of murine cardiac muscle changes in size across aging
Vue, Zer , Neikirk, Kit , Vang, Larry , Christensen, Trace , Shao, Jianqiang, Lam, Jacob , Marshall, Andrea G. , Crabtree, Amber , Anudokem, Josephs , Kirk, Benjamin , Bacevac, Serif, Barongan, Taylor , Shao, Bryanna, Stephens, Dominique C., Kabugi, Kinuthia, Koh, Ho‐Jin , Koh, Alice, Evans, Chantell S. , Reddy, Anilkumar K. , Actkins, Ky’Era V. , Zaganjor, Elma , Daneshgar, Nastaran , Murray, Sandra , Mobley, Bret C. , Gaddy, Jennifer A. , Riggs, Blake , McReynolds, Melanie R. , Gómez, José A. , Phillips, Mark , Exil, Vernat , Hinton, Antentor , Lam, Jacob , Beasley, Heather K , Marshall, Andrea G , Crabtree, Amber , Anudokem, Josephs , Barongan, Taylor , Evans, Chantell S , Daneshgar, Nastaran , Murray, Sandra A , Wanjalla, Celestine , Kirabo, Annet , Gomez, Jose A , Hinton, Antentor , Exil, Vernat , Garza-López, Edgar , Beasley, Heather K. , Rodríguez, Blanca , Kirk, Benjamin, Taylor, Brittany , Miller‐Fleming, Tyne W. , Damo, Steven M. , Wanjalla, Celestine N. , Dai, Dao‐Fu
American Journal of Physiology-Heart and Circulatory Physiology | August 25, 2023 | Volume: 325 | Issue: 5 | Pages: H965-H982
This article shows how mitochondria in murine cardiac changes, importantly elucidating age-related changes. It also is the first to show that the MICOS complex may play a role in outer membrane mitochondrial structure.
A Comprehensive Approach to Sample Preparation for Electron Microscopy and the Assessment of Mitochondrial Morphology in Tissue and Cultured Cells
Hinton, Antentor , Christensen, Trace , Mungai, Margaret , Shao, Jianqiang, Trushin, Sergey , Alghanem, Ahmad F. , Jaspersen, Adam, Geroux, Rachel E., Neikirk, Kit , Biete, Michelle , Shao, Bryanna, Vue, Zer , Vang, Larry , Beasley, Heather K. , Stephens, Dominique, Ponce, Jessica, Bleck, Christopher K. E. , Hicsasmaz, Innes, Murray, Sandra , Edmonds, Ranthony A. C. , Dajles, Andres , Koo, Young Do , Bacevac, Serif, Salisbury, Jeffrey L., Pereira, Renata O. , Trushina, Eugenia , Pereira, Renata O , Hinton, Antentor , Beasley, Heather K , Murray, Sandra A , Trushina, Eugenia , Katti, Prasanna , Zhang, Liang , Garza-López, Edgar , Marshall, Andrea G. , Stephens, Dominique C., Damo, Steven M. , Glancy, Brian , Abel, E. Dale
Advanced Biology | May 4, 2023 | Volume: 7 | Issue: 10 | Pages: No page available
Abstract Mitochondria respond to metabolic demands of the cell and to incremental damage, in part, through dynamic structural changes that include fission (fragmentation), fusion (merging of distinct mitochondria), autophagic degradation (mitophagy), and biogenic interactions with the endoplasmic reticulum (ER). High resolution study of mitochondrial structural and functional relationships requires rapid preservation of specimens to reduce technical artifacts coupled with quantitative assessment of mitochondrial architecture. A practical approach for assessing mitochondrial fine structure using two dimensional and three dimensional high‐resolution electron microscopy is presented, and a systematic approach to measure mitochondrial architecture, including volume, length, hyperbranching, cristae morphology, and the number and extent of interaction with the ER is described. These methods are used to assess mitochondrial architecture in cells and tissue with high energy demand, including skeletal muscle cells, mouse brain tissue, and Drosophila muscles. The accuracy of assessment is validated in cells and tissue with deletion of genes involved in mitochondrial dynamics.