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Streeter, Jennifer

https://orcid.org/0000-0002-6965-9546

Mayo Clinic

University of Iowa

Citations 2

Correction: Garza-Lopez et al. Protocols for Generating Surfaces and Measuring 3D Organelle Morphology Using Amira. Cells 2022, 11, 65
Vue, Zer , Katti, Prasanna , Neikirk, Kit , Biete, Michelle , Lam, Jacob , Beasley, Heather K. , Rodman, Taylor, Christensen, Trace , Salisbury, Jeffrey L., Vang, Larry , Mungai, Margaret , AshShareef, Salma, Murray, Sandra , Shao, Jianqiang, Pereira, Renata O. , Hinton, Antentor , Katti, Prasanna , Biete, Michelle , Beasley, Heather K , Murray, Sandra A , Pereira, Renata O , Hinton, Antentor , Garza-López, Edgar , Marshall, Andrea G. , Streeter, Jennifer , Glancy, Brian , Abel, E. Dale

Cells | May 11, 2023 | Volume: 12 | Issue: 10 | Pages: 1356

10.3390/cells12101356 Get It

In the original publication [...]
Protocols for Generating Surfaces and Measuring 3D Organelle Morphology Using Amira
Garza-López, Edgar , Vue, Zer , Katti, Prasanna , Neikirk, Kit , Biete, Michelle , Lam, Jacob , Beasley, Heather K. , Marshall, Andrea G. , Rodman, Taylor, Christensen, Trace , Salisbury, Jeffrey L., Vang, Larry , Mungai, Margaret , AshShareef, Salma, Murray, Sandra , Shao, Jianqiang, Streeter, Jennifer , Pereira, Renata O. , Hinton, Antentor , Pereira, Renata O , Hinton, Antentor , Katti, Prasanna , Beasley, Heather K , Murray, Sandra A , Lam, Jacob , Marshall, Andrea G , Glancy, Brian , Abel, E. Dale

Cells | December 28, 2021 | Volume: 11 | Issue: 1 | Pages: 65

10.3390/cells11010065 Get It

High-resolution 3D images of organelles are of paramount importance in cellular biology. Although light microscopy and transmission electron microscopy (TEM) have provided the standard for imaging cellular structures, they cannot provide 3D images. However, recent technological advances such as serial block-face scanning electron microscopy (SBF-SEM) and focused ion beam scanning electron microscopy (FIB-SEM) provide the tools to create 3D images for the ultrastructural analysis of organelles. Here, we describe a standardized protocol using the visualization software, Amira, to quantify organelle morphologies in 3D, thereby providing accurate and reproducible measurements of these cellular substructures. We demonstrate applications of SBF-SEM and Amira to quantify mitochondria and endoplasmic reticulum (ER) structures.