Citations 12
Upregulation of Endothelin-1 May Predict Chemotherapy-Induced Cardiotoxicity in Women with Breast Cancer
Krishnarao, Krithika , Bruno, Katelyn A. , Florio, Damian N. Di , Edenfield, Brandy , Whelan, Emily R. , Macomb, Logan P. , McGuire, Molly M., Hill, Anneliese R., Ray, Jordan , Cornell, Lauren , Tan, Winston , Geiger, Xochiquetzal J., Salomon, Gary R., Douglass, Erika J. , Fairweather, DeLisa , Yamani, Mohamad H. , Watkins, Molly M. , Di Florio, Damian N, Edenfield, Brandy H, Fairweather, DeLisa , Yamani, Mohamad H , Bruno, Katelyn A , Di Florio, Damian N , McGuire, Molly M., Cornell, Lauren F
Journal of Clinical Medicine | June 21, 2022 | Volume: 11 | Issue: 12 | Pages: 3547
As survival in breast cancer patients from newer therapies increases, concerns for chemotherapy-induced cardiotoxicity (CIC) have offset some of these benefits, manifesting as a decline in left ventricular ejection fraction (LVEF). Patients receiving anthracycline-based chemotherapy followed by trastuzumab are at risk for CIC. Previous research evaluating whether clinical biomarkers predict cardiotoxicity has been inconsistent. Recently, angiotensin II type 1 receptor (ATR1) and endothelin 1 (ET1) have been shown to play a role in breast tumor growth. We evaluated ATR1 and ET1 expression in breast cancer tissue and its association with CIC. A total of 33 paraffin-embedded breast tissue specimens from women with breast cancer treated with anthracycline-based chemotherapy and trastuzumab were analyzed by immunohistochemistry (IHC) and qRT-PCR. We found that ET1 expression was increased in patients with an LVEF ≤ 50% (p = 0.032) with a lower LVEF correlating with higher ET1 expression (r = 0.377, p = 0.031). In patients with a change in LVEF of greater than 10%, greater ET1 expression was noted compared to those without a change in LVEF (p = 0.017). Increased ET1 expression in breast tumor tissue is associated with reduced LVEF. Future studies need to examine whether ET1 may be a tissue biomarker that helps predict the risk of developing CIC in women with breast cancer.
Sex-Specific Effects of Plastic Caging in Murine Viral Myocarditis
Bruno, Katelyn A. , Macomb, Logan P. , Morales-Lara, Andrea Carolina , Mathews, Jessica, Frisancho, J. Augusto, Yang, Alex Lingyun, Florio, Damian N. Di , Edenfield, Brandy , Whelan, Emily R. , Salomon, Gary R., Hill, Anneliese R., Hewa‐Rahinduwage, Chathuranga C., Scott, Ashley Jennie , Greyner, Henry, Molina, Frank, Greenaway, Merci S., Cooper, George , Fairweather, DeLisa , Morales-Lara, A Carolina, Mathews, Jessica E, Yang, Alex L, Di Florio, Damian N, Edenfield, Brandy H, Fairweather, DeLisa , Bruno, Katelyn A , Di Florio, Damian N , Cooper, George M.
International Journal of Molecular Sciences | August 17, 2021 | Volume: 22 | Issue: 16 | Pages: 8834
Background: Myocarditis is an inflammatory heart disease caused by viral infections that can lead to heart failure, and occurs more often in men than women. Since animal studies have shown that myocarditis is influenced by sex hormones, we hypothesized that endocrine disruptors, which interfere with natural hormones, may play a role in the progression of the disease. The human population is exposed to the endocrine disruptor bisphenol A (BPA) from plastics, such as water bottles and plastic food containers. Methods: Male and female adult BALB/c mice were housed in plastic versus glass caging, or exposed to BPA in drinking water versus control water. Myocarditis was induced with coxsackievirus B3 on day 0, and the endpoints were assessed on day 10 post infection. Results: We found that male BALB/c mice that were exposed to plastic caging had increased myocarditis due to complement activation and elevated numbers of macrophages and neutrophils, whereas females had elevated mast cell activation and fibrosis. Conclusions: These findings show that housing mice in traditional plastic caging increases viral myocarditis in males and females, but using sex-specific immune mechanisms.