Citations 3
The Promise and Pitfalls of PRAME Immunohistochemistry During Mohs Surgery: Early Findings
Lohman, Mary E. , Steen, A. , Demer, Addison M. , Cervenka, Derek J., Vidal, Nahid Y. , Kim, Yong-Hun , Cervenka, Derek J.
The American Journal of Dermatopathology | January 26, 2024 | Volume: 46 | Issue: 3 | Pages: 191-192
Utility of Intraoperative Cytokeratin-7 Immunostaining During Mohs Micrographic Surgery for Sebaceous Carcinoma
Steen, A. , Demer, Addison M. , Vidal, Nahid Y. , Crum, Olivia M.
Dermatologic Surgery | January 23, 2024 | Volume: 50 | Issue: 4 | Pages: 384-386
The utility of PRAME staining in identifying malignant transformation of melanocytic nevi
Lohman, Mary E. , Steen, A. , Grekin, Roy C. , North, Jeffrey P. , Lohman, Mary E. , Steen, Aaron J
Journal of Cutaneous Pathology | January 15, 2021 | Volume: 48 | Issue: 7 | Pages: 856-862
AbstractBackgroundPReferentially expressed Antigen in MElanoma (PRAME) immunohistochemical (IHC) staining is used to aid melanoma diagnosis. PRAME expression in nevus‐associated melanoma (NAM) has not been evaluated.MethodsPRAME IHC was applied to cases of NAM; staining for each population of melanocytes (benign and malignant) was graded based on the percentage of labeled cells. No labeling was graded 0, 1% to 25% labeling was 1+, 26% to 50% was 2+, 51% to 75% was 3+, and >76% was 4+.ResultsThirty‐six cases were reviewed. Sixty‐seven percent (24/36) of melanomas were PRAME positive (4+) while no (0/36) nevi showed 4+ positivity. Eighty‐one percent (29/36) of nevi were completely PRAME negative compared to 17% (6/36) of melanomas. In 67% of cases (24/36) PRAME differentiated between benign and malignant melanocyte populations.ConclusionsWe identified a high rate (67%) of differential PRAME staining in adjacent benign and malignant melanocyte populations in NAM. In PRAME positive (4+) melanomas, PRAME differentiates 100% (24/24) of benign and malignant melanocyte populations. When 4+ staining is used as the threshold for positivity, PRAME staining has a sensitivity of 67% (24/36) and a specificity of 100% (36/36). These results support PRAME IHC can assist in distinguishing melanocyte populations in melanoma arising within nevi.