Citations 1
Targeted Metagenomics for Clinical Detection and Discovery of Bacterial Tick-Borne Pathogens
Kingry, Luke C. , Sheldon, Sarah W., Oatman, Stephanie, Pritt, Bobbi S. , Anacker, Melissa , Bjork, Jenna , Neitzel, David F., Strain, Anna K., Berry, Jon, Sloan, Lynne M., Respicio-Kingry, Laurel, Dietrich, Elizabeth A. , Bloch, Karen C. , Moncayo, Abelardo C., Srinivasamoorthy, Ganesh, Hu, Bin , Mead, Paul S. , Petersen, Jeannine M., Kingry, Luke , Pritt, Bobbi, Sloan, Lynne, Pritt, Bobbi S , Berry, Jonathan, Hinckley, Alison F. , Kugeler, Kiersten J.
Journal of Clinical Microbiology | September 1, 2020 | Volume: 58 | Issue: 11 | Pages: No page available
Tick-borne diseases, due to a diversity of bacterial pathogens, represent a significant and increasing public health threat throughout the Northern Hemisphere. A high-throughput 16S V1-V2 rRNA gene-based metagenomics assay was developed and evaluated using >13,000 residual samples from patients suspected of having tick-borne illness and >1,000 controls. Taxonomic predictions for tick-borne bacteria were exceptionally accurate, as independently validated by secondary testing. Overall, 881 specimens were positive for bacterial tick-borne agents.