Citations 1
Small Interfering RNA Screening for the Small GTPase Rab Proteins Identifies Rab5B as a Major Regulator of Hepatitis B Virus Production
Inoue, Jun , Ninomiya, Masashi , Umetsu, Teruyuki, Nakamura, Takuya , Kogure, Takayuki , Kakazu, Eiji , Iwata, Tomoaki, Takai, Satoshi, Sano, Akitoshi , Fukuda, Mitsunori , Watashi, Koichi , Isogawa, Masanori , Tanaka, Yasuhito , Shimosegawa, Tooru , McNiven, Mark A. , Masamune, Atsushi
Journal of Virology | May 22, 2019 | Volume: 93 | Issue: 15 | Pages: No page available
HBV infection is a worldwide health problem, but the mechanisms of how HBV utilizes cellular machinery for its life cycle are poorly understood. In particular, it has been unclear how the viral components and virions are transported among the organelles. The HBV budding site has been reported to be the ER or MVB, but it has not been clearly determined. In this study, siRNA-based screening of Rab proteins using HBV-expressing cells showed that Rab5B, one of the Rab5 isoforms, has important roles in late steps of the HBV life cycle. Although Rab5 is known to work on early endosomes, this study showed that Rab5B plays a role in the transport of LHBs between the ER and MVB. Furthermore, it affects the transcription of LHBs. This is the first report on the mechanisms of HBV envelope protein transport among the organelles, and the results provide important insights into the therapeutic control of HBV infection.