Citations 2
Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation
Tian, Ming , Ticer, Taylor , Walker, Sierra A. , Pham, Anthony , Suh, Annie, Busatto, Sara , Davidovich, Irina, Al‐kharboosh, Rawan , Lewis‐Tuffin, Laura J. , Ji, Baoan , Quiñones‐Hinojosa, Alfredo , Rückert, Felix , Wolfram, Joy , Wang, Qikun, Walker, Sierra, Al-Kharboosh, Rawan, Lewis-Tuffin, Laura, Wolfram, Joy , Wang, Qikun , Talmon, Yeshayahu , Shapiro, Shane A.
Small | February 18, 2020 | Volume: 16 | Issue: 10 | Pages: No page available
Abstract Extracellular vesicles secreted from adipose‐derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti‐inflammatory properties of adipose tissue‐derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC‐EVs, lipoaspirate nanoparticles (Lipo‐NPs) take less time to process (hours compared to months) and cost less to produce (clinical‐grade cell culture facilities are not required). The physicochemical characteristics and anti‐inflammatory properties of Lipo‐NPs are evaluated and compared to those of patient‐matched ADSC‐EVs. Moreover, guanabenz loading in Lipo‐NPs is evaluated for enhanced anti‐inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo‐NPs compared to ADSC‐EVs. Additionally, the uptake of Lipo‐NPs in hepatocytes and macrophages is higher. Lipo‐NPs and ADSC‐EVs have comparable protective and anti‐inflammatory effects. Specifically, Lipo‐NPs reduce toll‐like receptor 4‐induced secretion of inflammatory cytokines in macrophages. Guanabenz‐loaded Lipo‐NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.
Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid
Busatto, Sara , Vilanilam, George K. , Ticer, Taylor , Lin, Wen‐Lang, Shapiro, Shane A. , Bergese, Paolo , Wolfram, Joy , Vilanilam, George , Shapiro, Shane , Bergese, Paolo , Wolfram, Joy , Dickson, Dennis W.
Cells | December 18, 2018 | Volume: 7 | Issue: 12 | Pages: 273
Concentration of extracellular vesicles (EVs) from biological fluids in a scalable and reproducible manner represents a major challenge. This study reports the use of tangential flow filtration (TFF) for the highly efficient isolation of EVs from large volumes of samples. When compared to ultracentrifugation (UC), which is the most widely used method to concentrate EVs, TFF is a more efficient, scalable, and gentler method. Comparative assessment of TFF and UC of conditioned cell culture media revealed that the former concentrates EVs of comparable physicochemical characteristics, but with higher yield, less single macromolecules and aggregates (<15 nm in size), and improved batch-to-batch consistency in half the processing time (1 h). The TFF protocol was then successfully implemented on fluids derived from patient lipoaspirate. EVs from adipose tissue are of high clinical relevance, as they are expected to mirror the regenerative properties of the parent cells.