Greenleaf, James F.

Vibro-acoustography (VA) is an imaging method based on ultrasound radiation force. In this method, a two dimensional image is formed by scanning two co-focused ultrasound beams with slightly different frequencies over the object (biological tissue) in a C-scan format and recording resulting sound that is emitted from the focal region at the difference frequency. As in other ultrasound-based imaging modalities, it is important to study the thermal safety of the technique. This paper addresses tissue heating due to a VA scan using a concentric confocal transducer. The three dimensional ultrasound intensity field calculated by Field II is used with the bio-heat equation to estimate tissue heating. Results calculated with thermal conduction and with blood perfusion, with conduction and without perfusion, and without conduction and without perfusion are compared. Maximum temperature rise occurs in the transducer near field during VA scans. Heating during one VA scan is below 0.05 °C for soft tissue with attenuation coefficient of 0.3, 0.5, or 0.7 dB/cm MHz. It is concluded that temperature rise in tissue is not significant. [Work supported by NIH Grants CA 91956, CA 127235, and CA121579. Some of the techniques are patented by Mayo Clinic and the some of the authors (M.F. and J.F.G.).]

The mechanical response of tissues to external forces has gained considerable interest in medical diagnosis. Typically, a force is needed to produce displacement in the tissue for estimation of the tissue’s elasticity. One approach is to apply an external force on the body’s surface. Another emerging technique is to generate a localized force inside the tissue with ultrasound. Shear waves can be used in bulky tissues for estimating the tissue’s elasticity. The bending wave can be generated and measured in vessels for noninvasive estimation of a vessel’s elasticity. We have found that the Rayleigh surface wave can be generated on the tissue’s surface, [X. Zhang et al., Proc. International Congress on Ultrasonics, Vienna, April 9–13, 2007], [X. Zhang et al., IEEE Trans. Medical Imaging, 26, 843–852 (2007)]. In our method, a localized radiation force of ultrasound is remotely and noninvasively applied inside the tissue. This force can generate the shear wave as well as compression wave inside the tissue. However, only the surface wave can exist on the body’ surface. The surface wave can be used to estimate and image the stiffness of tissue. Some results of using the surface wave are presented.

Thyroid imaging with conventional ultrasound scanners does not always lead to conclusive results. Furthermore, current ultrasound imaging technology has difficulty in detecting important microcalcifications. Ambiguities in ultrasound and other thyroid imaging methods lead to a large number of unnecessary biopsies. For these reasons, alternative imaging methods capable of detecting both microcalcifications and lesions in thyroid are of great interest. This paper describes recent results on imaging human thyroids with vibro-acoustography. Experiments were conducted on eight excised human thyroids from autopsy. The specimens were each imbedded in a block of gel to simulate the surrounding tissue and to facilitate scanning. Three types of images were acquired from each specimen: X-ray, B-mode ultrasound, and vibro-acoustography. Anatomical details seen in the images were correlated and image quality was compared. X-ray images displayed calcifications with high contrast, but did not show anatomical details of soft tissue. B-mode displayed some soft tissue structures; however, the speckle masked the details and reduced the contrast. Vibro-acoustography displayed calcifications, anatomical details, and some nodules where they existed. Furthermore, vibro-acoustography images displayed tissue structures with high contrast and free from speckle. It is concluded that vibro-acoustography may be a suitable technique for thyroid imaging.

Modulating ultrasound can create dynamic ultrasound radiation force to induce local tissue vibration. Using different types of modulating signals produces radiation force with weighted multifrequency components. Modulation signals with frequency fr produce force with components at multiples of fr. Both amplitude modulation (AM) and double sideband suppressed carrier (DSB-SC) amplitude modulation were explored. Different waveforms were investigated such as sine, square, triangle, and sawtooth for modulating continuous wave ultrasound. A 3.0 MHz transducer produced ultrasound which was measured with a needle hydrophone and used to derive and analyze the spectrum of the radiation force function. The modulated ultrasound was used to vibrate a steel sphere in a gelatin phantom, and the motion was measured using a laser vibrometer. The measured spectra were used to find the frequency response of the sphere. Different modulating signals were used to produce multifrequency radiation force. The sphere frequency response was recovered using different modulating signals. Weighted multifrequency radiation force was produced by modulating ultrasound with different signals. Application of multifrequency radiation force can be used to measure the frequency response of objects or tissue. [This work was supported in part by grants EB002167, EB002640, and CA091956 from NIH.]

A new method for imaging and detecting modal shapes of vessels is introduced. Theory is developed that predicts the measured velocity is proportional to the value of the mode shape at the focal point of the ultrasound beam. Experimental a cylindrical gel phantom of large radius. This model simulates approximately a large artery and the surrounding body. The fundamental frequency was measured 83 Hz for the tube-phantom system. At this frequency the ultrasound transducer was scanned across the vessel plane with velocity measurement at one single point on the vessel and on the phantom by laser. The images obtained show clearly the interior tube and the modal shape of the tube.

Vibro-acoustography is a new noncontact imaging method based on the radiation force of ultrasound. We extend this new technique for tissue characterization of arterial vessels by vibration techniques. In this paper a theoretical model for vibration analysis of arterial vessel with tissue is developed. Experimental studies were carried out on a silicone rubber tube embedded in a cylindrical gelatin phantom of larger radius, which simulates a large artery and the tissue body. The fundamental mode is well excited by the radiation force of ultrasound. The fundamental frequency was measured 81.8 Hz for a tube-phantom structure that is quite close to our theoretical prediction of 83.3 Hz.

Considerable interest in the elastic properties of soft tissue has increased in medicine due to their clinical relevance for monitoring various diseases as well as a biomarker for cancer. A recent emerging field is the use of acoustic radiation force for imaging and estimating the elastic properties of tissue. Newly developed imaging modalities include shear wave elasticity imaging, supersonic shear wave imaging, acoustic radiation force impulse imaging, and vibro-acoustography. In these new methods, the shear wave is used to estimate the elasticity of tissue. We have developed a novel surface wave method for noninvasively estimating the elasticity of tissue [X. Zhang et al., J. Acoust. Soc. Am., 122, 2522-2525 (2007)]. In this method, a localized ultrasound radiation force is remotely and noninvasively applied inside the tissue. The surface wave speed is used to estimate the elasticity of tissue. This method has been further developed and applied to estimate the viscoelasticity of animal myocardium tissue. In this abstract, we report our recent results of in vitro studies on animal myocardium tissue. Both the surface wave and shear wave are measured and analyzed. Noninvasive characterization of viscoelastic properties of myocardium tissue may be a very important tool for assessing heart function.

Harmonic or pulsed radiation pressure and subsequent measurement of the tissue response can be used to accurately quantitatively measure fundamental material properties of tissue. The measurement of shear wave dispersion can be used to estimate elastic shear moduli of tissue using an appropriate model. Fundamental model free properties such as complex modulus can also be measured. Ultrasound radiation pressure is used to induce free propagating shear waves. The measurable properties of the shear waves such as speed are sensitive to only the local material properties of the tissue under certain circumstances. A model relating the shear wave speed as a function of frequency is related to the elastic and viscous moduli within small regions of tissue according to, for instance, the Voigt model. Shear and elastic moduli in tissue are measured with high accuracy and precision given appropriate models of wave propagation within the geometry of the tissue. Complex modulus in liver of live pig and other tissues have been made. Careful use of shear wave propagation and subsequent measurements can provide fundamental quantitative measurements of tissue mechanical properties if models are accurate. An application of this method is the noninvasive measurement of liver stiffness as a surrogate for fibrosis.

High-resolution ultrasound imaging systems for inspection of defects and flaws in materials are of great demand in many industries. Among these systems, Vibro-acoustography (VA) has shown excellent capabilities as a non-contact method for non-destructive high-resolution imaging applications. This method consists of mixing two confocal ultrasound beams, slightly shifted in frequency, to produce an acoustic emission field at the difference frequency of the primary incident ultrasound beams. In addition to the difference frequency signal, there exists another signal at the sum frequency, formed in the intersection region of the two primary beams. The goal of this study is to investigate the formation of high-resolution images using the sum frequency of ultrasound waves in VA while concurrently forming the conventional difference-frequency VA image, thereby increasing the amount of information acquired during a single scan. A theoretical model describing the sum frequency wave propagation, including beam forming and image formation in the confocal configuration is developed and verified experimentally. Moreover, sample experiments are performed on a flawed fiber-reinforced ceramic composite plate. Images at both the difference and sum frequencies are compared and discussed. Results show that the sum frequency image produces a high-resolution C-scan of the plate by which the flaws and structural details of the plate can be detected.