Citations 11
Identification of a Novel Tumor Suppressor Gene p34 on Human Chromosome 6q25.1
Vikis, Haris G., Wang, Yian , Jia, Dongmei , Wang, Daolong, Bailey‐Wilson, Joan E. , Pinney, Susan M. , Petersen, Gloria M. , Wiest, Jonathan S., Fain, Pamela R., Schwartz, Ann G. , Gazdar, Adi F., Gaba, Colette , Rothschild, Henry, Mandal, Diptasri , Kupert, Elena, Seminara, Daniela, Liu, Yan , Viswanathan, Avinash, Govindan, Ramaswamy , Anderson, Marshall W., You, Ming , Wang, Min , Bierut, Laura Jean , Amos, Christopher I. , Andrade, Mariza de , Yang, Ping , Gazdar, Adi, Minna, John Dorrance , Wilson, Richard K , Pinney, Susan M , Govindan, Ramaswamy
Cancer Research | January 8, 2007 | Volume: 67 | Issue: 1 | Pages: 93-99
Abstract In this study, we observed loss of heterozygosity (LOH) in human chromosomal fragment 6q25.1 in sporadic lung cancer patients. LOH was observed in 65% of the 26 lung tumors examined and was narrowed down to a 2.2-Mb region. Single-nucleotide polymorphism (SNP) analysis of genes located within this region identified a candidate gene, termed p34. This gene, also designated as ZC3H12D, C6orf95, FLJ46041, or dJ281H8.1, carries an A/G nonsynonymous SNP at codon 106, which alters the amino acid from lysine to arginine. Nearly 73% of heterozygous lung cancer tissues with LOH and the A/G SNP also exhibited loss of the A allele. In vitro clonogenic and in vivo nude mouse studies showed that overexpression of the A allele exerts tumor suppressor function compared with the G allele. p34 is located within a recently mapped human lung cancer susceptibility locus, and association of the p34 A/G SNP was tested among these families. No significant association between the less frequent G allele and lung cancer susceptibility was found. Our results suggest that p34 may be a novel tumor suppressor gene involved in sporadic lung cancer but it seems not to be the candidate familial lung cancer susceptibility gene linked to chromosomal region 6q23-25. [Cancer Res 2007;67(1):93–9]